Microstructured arrays based on self-assembled fibrillar networks for specific cell immobilization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F22%3A63557698" target="_blank" >RIV/70883521:28610/22:63557698 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28110/22:63557698

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Microstructured arrays based on self-assembled fibrillar networks for specific cell immobilization

Popis výsledku v původním jazyce

We established DNA–spider silk conjugates to create hybrid materials. The spider silk moiety enabled self-assembly into nanofibrils controlled by phosphate ions in aqueous buffers, whilst the DNA part enabled DNA-specific fibril labeling or sequence-specific immobilization of DNA–spider silk conjugates. Recently, we developed surface nucleated self-assembly of the DNA-modified recombinant spider silk proteins into immobilized fibril-based networks, which revealed swelling and softening properties of nanohydrogels . The DNA functionalization in the highly hydrated cell repellent networks could be used for a specific cell binding. For this, a mild lipid–DNA incorporation into the cell membrane was employed, which showed high labelling efficiency as well as negligible cytotoxicity. Based on the complementarity of the nucleic acids, highly specific DNA-assisted immobilization of the cells on the nanohydrogels with tuneable cell densities was possible. The designed competitor DNA probes enabled the cell’s liftoff on demand. We also employed modification of the nanohydrogels with DNA aptamers capable of binding to cell markers to immobilize different types of cancer cells specifically. The principle of surface-nucleated nanohydrogel self-assembly was further combined with photolithography. Using a positive-tone resist on an amino-reactive substrate, arbitrarily shaped microwells were patterned, the bottom of which served to define the binding of spider silk nucleation sites and the formation of the fibrillar networks. After stripping the sacrificial photoresists, a nanohydrogel micro-structured pattern was achieved, enabling either the DNA-assisted immobilization of DNA-labelled cells or the aptamer–cancer cell marker interactions with high spatial fidelity

Název v anglickém jazyce

Microstructured arrays based on self-assembled fibrillar networks for specific cell immobilization

Popis výsledku anglicky

We established DNA–spider silk conjugates to create hybrid materials. The spider silk moiety enabled self-assembly into nanofibrils controlled by phosphate ions in aqueous buffers, whilst the DNA part enabled DNA-specific fibril labeling or sequence-specific immobilization of DNA–spider silk conjugates. Recently, we developed surface nucleated self-assembly of the DNA-modified recombinant spider silk proteins into immobilized fibril-based networks, which revealed swelling and softening properties of nanohydrogels . The DNA functionalization in the highly hydrated cell repellent networks could be used for a specific cell binding. For this, a mild lipid–DNA incorporation into the cell membrane was employed, which showed high labelling efficiency as well as negligible cytotoxicity. Based on the complementarity of the nucleic acids, highly specific DNA-assisted immobilization of the cells on the nanohydrogels with tuneable cell densities was possible. The designed competitor DNA probes enabled the cell’s liftoff on demand. We also employed modification of the nanohydrogels with DNA aptamers capable of binding to cell markers to immobilize different types of cancer cells specifically. The principle of surface-nucleated nanohydrogel self-assembly was further combined with photolithography. Using a positive-tone resist on an amino-reactive substrate, arbitrarily shaped microwells were patterned, the bottom of which served to define the binding of spider silk nucleation sites and the formation of the fibrillar networks. After stripping the sacrificial photoresists, a nanohydrogel micro-structured pattern was achieved, enabling either the DNA-assisted immobilization of DNA-labelled cells or the aptamer–cancer cell marker interactions with high spatial fidelity

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Projekt

<a href="/cs/project/GA22-33307S" target="_blank" >GA22-33307S: Vývoj nových 3D hierarchicky strukturovaných polysacharidových a proteinových porézních systémů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů