Acinetobacter pragensis sp nov., found in soil and water ecosystems

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F16%3A00011560" target="_blank" >RIV/75010330:_____/16:00011560 - isvavai.cz</a>

Výsledek na webu

<a href="http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001285" target="_blank" >http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001285</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/ijsem.0.001285" target="_blank" >10.1099/ijsem.0.001285</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Acinetobacter pragensis sp nov., found in soil and water ecosystems

Popis výsledku v původním jazyce

This study aimed to define the taxonomic status of a novel, phenetically distinct group of seven strains belonging to the genus Acinetobacter, which were isolated from environmental soil and water samples collected in Central Bohemia, Czech Republic. Comparative sequence analyses of the 16S rRNA, gyrB and rpoB genes showed that all these strains formed respective tight clusters ( intracluster sequence similarities of at least 99.8, at least 98.1 and at least 98.3 %, respectively), which were distant from all known Acinetobacter species (at most 98.2, at most 84.0 and at most 88.9 %, respectively). The average nucleotide identity and digital DNA-DNA hybridization values (at most 83.5 and at most 27.4 %, respectively) between the whole-genome sequence of a group representative (strain ANC 4149(T)) and those of known taxa were far below the thresholds used to discriminate between bacterial species. The seven strains also formed a tight and distinct cluster based on the genus-wide comparison of whole-cell mass fingerprints generated by matrix-assisted laser desorption/ionization time-of-flight MS and could be distinguished from all other members of the genus Acinetobacter by the combination of their ability to assimilate glutarate and L-tartrate and inability to grow at 37 degrees C and on L-aspartate. It is concluded that the seven strains represent a novel species for which the name Acinetobacter pragensis sp. nov. is proposed. The type strain is ANC 4149(T) (= CCM 8637(T) = CCUG 67962(T) = CNCTC 7530(T)).

Název v anglickém jazyce

Acinetobacter pragensis sp nov., found in soil and water ecosystems

Popis výsledku anglicky

This study aimed to define the taxonomic status of a novel, phenetically distinct group of seven strains belonging to the genus Acinetobacter, which were isolated from environmental soil and water samples collected in Central Bohemia, Czech Republic. Comparative sequence analyses of the 16S rRNA, gyrB and rpoB genes showed that all these strains formed respective tight clusters ( intracluster sequence similarities of at least 99.8, at least 98.1 and at least 98.3 %, respectively), which were distant from all known Acinetobacter species (at most 98.2, at most 84.0 and at most 88.9 %, respectively). The average nucleotide identity and digital DNA-DNA hybridization values (at most 83.5 and at most 27.4 %, respectively) between the whole-genome sequence of a group representative (strain ANC 4149(T)) and those of known taxa were far below the thresholds used to discriminate between bacterial species. The seven strains also formed a tight and distinct cluster based on the genus-wide comparison of whole-cell mass fingerprints generated by matrix-assisted laser desorption/ionization time-of-flight MS and could be distinguished from all other members of the genus Acinetobacter by the combination of their ability to assimilate glutarate and L-tartrate and inability to grow at 37 degrees C and on L-aspartate. It is concluded that the seven strains represent a novel species for which the name Acinetobacter pragensis sp. nov. is proposed. The type strain is ANC 4149(T) (= CCM 8637(T) = CCUG 67962(T) = CNCTC 7530(T)).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA13-26693S" target="_blank" >GA13-26693S: Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Systematic and Evolutionary Microbiology

ISSN

1466-5026

e-ISSN

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Svazek periodika

66

Číslo periodika v rámci svazku

Pt. 10

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

3897-3903

Kód UT WoS článku

000386871600022

EID výsledku v databázi Scopus

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