Raman label-free visualisation of titanium dioxide nanoparticles uptake in BJ cell lines

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F16%3A00011654" target="_blank" >RIV/75010330:_____/16:00011654 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/16:33160603

Výsledek na webu

<a href="http://www.prolekare.cz/lekar-a-technika-clanek/raman-label-free-visualisation-of-titanium-dioxide-nanoparticles-uptake-in-bj-cell-lines-59422" target="_blank" >http://www.prolekare.cz/lekar-a-technika-clanek/raman-label-free-visualisation-of-titanium-dioxide-nanoparticles-uptake-in-bj-cell-lines-59422</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Raman label-free visualisation of titanium dioxide nanoparticles uptake in BJ cell lines

Popis výsledku v původním jazyce

Titanium dioxide nanoparticles represent one of the most frequently applied nanomaterials. Due to its advantageous physicochemical properties affecting the final products, use of this nanomaterial in daily used products is increasing. Beside the addition into glaze or enamels, titanium dioxide nanoparticles are found in UV protective cosmetic products applied on skin. According to the studies confirming the potential carcinogenic effect of titanium dioxide nanoparticles application of such nanomaterial may cause health risk. Cellular uptake of nanoparticles and their distribution in cell environment may play an important role in nanoparticles toxicological effect. Thus, evaluation of cellular uptake of nanoparticles is the additional step for evaluation of nanoparticles toxicology. The main objective of this study was to confirm the assumption of the cellular uptake of tested TiO2 nanoparticles using human fibroblasts BJ cell lines and confocal Raman microscopy as a new, promising, label-free imaging technique for studying the distribution of exogenous substances in cells. The results of this study confirm that tested TiO2 nanoparticles are uptaken by cells and distributed in intracellular environment, where form aggregates, possibly during their transport via endocytosis.

Název v anglickém jazyce

Raman label-free visualisation of titanium dioxide nanoparticles uptake in BJ cell lines

Popis výsledku anglicky

Titanium dioxide nanoparticles represent one of the most frequently applied nanomaterials. Due to its advantageous physicochemical properties affecting the final products, use of this nanomaterial in daily used products is increasing. Beside the addition into glaze or enamels, titanium dioxide nanoparticles are found in UV protective cosmetic products applied on skin. According to the studies confirming the potential carcinogenic effect of titanium dioxide nanoparticles application of such nanomaterial may cause health risk. Cellular uptake of nanoparticles and their distribution in cell environment may play an important role in nanoparticles toxicological effect. Thus, evaluation of cellular uptake of nanoparticles is the additional step for evaluation of nanoparticles toxicology. The main objective of this study was to confirm the assumption of the cellular uptake of tested TiO2 nanoparticles using human fibroblasts BJ cell lines and confocal Raman microscopy as a new, promising, label-free imaging technique for studying the distribution of exogenous substances in cells. The results of this study confirm that tested TiO2 nanoparticles are uptaken by cells and distributed in intracellular environment, where form aggregates, possibly during their transport via endocytosis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

DN - Vliv životního prostředí na zdraví

OECD FORD obor

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Projekt

<a href="/cs/project/LO1304" target="_blank" >LO1304: Podpora udržitelnosti Ústavu molekulární a translační medicíny</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Lékař a technika

ISSN

0301-5491

e-ISSN

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Svazek periodika

46

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

25-28

Kód UT WoS článku

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EID výsledku v databázi Scopus

2-s2.0-84997427321