Acinetobacter colistiniresistens sp nov (formerly genomic species 13 sensu Bouvet and Jeanjean and genomic species 14 sensu Tjernberg and Ursing), isolated from human infections and characterized by intrinsic resistance to polymyxins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F17%3A00011839" target="_blank" >RIV/75010330:_____/17:00011839 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/17:00095314

Výsledek na webu

<a href="http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001903" target="_blank" >http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001903</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/ijsem.0.001903" target="_blank" >10.1099/ijsem.0.001903</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Acinetobacter colistiniresistens sp nov (formerly genomic species 13 sensu Bouvet and Jeanjean and genomic species 14 sensu Tjernberg and Ursing), isolated from human infections and characterized by intrinsic resistance to polymyxins

Popis výsledku v původním jazyce

Strains of the genus Acinetobacter, classified as genomic species 13BJ/14TU have been previously associated with human infections and resistance to colistin. To clarify the taxonomy of this provisional group, we investigated 24 strains that have been isolated from humans since the 1960s in 10 countries. The genus-wide analysis of the rpoB and gyrB sequences of all strains and whole-genome sequences of strains representing different rpoB/gyrB genotypes showed that the 24 strains formed a distinct monophyletic group within the so-called haemolytic clade of the genus Acinetobacter. The distinctness of the group at the species level was supported by the results of the cluster analysis of the whole-cell protein fingerprints generated by matrix-assisted laser desorption ionization-time-of-flight MS. The 24 strains had very similar metabolic features and could be distinguished from other members of the genus by the combination of strong haemolytic and proteolytic activities and the ability to oxidize D-glucose and grow on phenylacetate and/or L-phenylalanine. The minimum inhibitory concentrations of the 24 strains to colistin and polymyxin B ranged from 16 to 64 mgl(-1) and from 4 to 32 mgl(-1), respectively, so uniformly reaching the current clinical resistance breakpoint (4mg l(-1)) for these drugs. Genus-wide comparison revealed that such a consistently high level of resistance to polymyxins is a unique feature among species of the genus Acinetobacter, which occur in humans. We conclude that genomic species 13BJ/14TU represents a biologically meaningful and medically relevant species, for which the name Acinetobacter colistiniresistens sp. nov. is proposed. The type strain is NIPH 2036(T) (=CCM 8641(T)=CIP 110478(T)=CCUG 67966(T)=CNCTC 7573(T)).

Název v anglickém jazyce

Acinetobacter colistiniresistens sp nov (formerly genomic species 13 sensu Bouvet and Jeanjean and genomic species 14 sensu Tjernberg and Ursing), isolated from human infections and characterized by intrinsic resistance to polymyxins

Popis výsledku anglicky

Strains of the genus Acinetobacter, classified as genomic species 13BJ/14TU have been previously associated with human infections and resistance to colistin. To clarify the taxonomy of this provisional group, we investigated 24 strains that have been isolated from humans since the 1960s in 10 countries. The genus-wide analysis of the rpoB and gyrB sequences of all strains and whole-genome sequences of strains representing different rpoB/gyrB genotypes showed that the 24 strains formed a distinct monophyletic group within the so-called haemolytic clade of the genus Acinetobacter. The distinctness of the group at the species level was supported by the results of the cluster analysis of the whole-cell protein fingerprints generated by matrix-assisted laser desorption ionization-time-of-flight MS. The 24 strains had very similar metabolic features and could be distinguished from other members of the genus by the combination of strong haemolytic and proteolytic activities and the ability to oxidize D-glucose and grow on phenylacetate and/or L-phenylalanine. The minimum inhibitory concentrations of the 24 strains to colistin and polymyxin B ranged from 16 to 64 mgl(-1) and from 4 to 32 mgl(-1), respectively, so uniformly reaching the current clinical resistance breakpoint (4mg l(-1)) for these drugs. Genus-wide comparison revealed that such a consistently high level of resistance to polymyxins is a unique feature among species of the genus Acinetobacter, which occur in humans. We conclude that genomic species 13BJ/14TU represents a biologically meaningful and medically relevant species, for which the name Acinetobacter colistiniresistens sp. nov. is proposed. The type strain is NIPH 2036(T) (=CCM 8641(T)=CIP 110478(T)=CCUG 67966(T)=CNCTC 7573(T)).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30303 - Infectious Diseases

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Systematic and Evolutionary Microbiology

ISSN

1466-5026

e-ISSN

1466-5034

Svazek periodika

67

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

2134-2141

Kód UT WoS článku

000408263300010

EID výsledku v databázi Scopus

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