Lokalizace proteinů prasečí semenné plasmy (PSP) v kančím reprodukčním traktu a spermiích

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F08%3A00308662" target="_blank" >RIV/86652036:_____/08:00308662 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/08:00307374

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Localization of porcine seminal plasma (PSP) proteins in the boar reproductive tract and spermatozoa

Popis výsledku v původním jazyce

Boar spermadhesins (AQN, AWN, PSP) are proteins containing a characteristic CUB domain, originally isolated from seminal plasma and ejaculated spermatozoa, with various role in reproduction. Unlike AQN and AWN spermadhesins, localization of porcine seminal plasma (PSP) proteins in boar reproductive tract has not been completely resolved. This work is focused on PSP protein localization in the boar reproductive organs and on spermatozoa. Using specific polyclonal antibodies, PSP I and PSP II proteins were immunodetected in tissue extracts from cauda epididymis, prostate, seminal vesicles and Cowper´s glands on the blots, and in secretory tissues of these organs by immunofluorescence. The ability of PSP proteins to bind the epididymal spermatozoa indicated their presence on cauda epididymal and ejaculated spermatozoa. PSP proteins are produced not only by seminal vesicles and prostate, but also by epididymis. However, their prospective role in sperm epididymal maturation is not clear

Název v anglickém jazyce

Localization of porcine seminal plasma (PSP) proteins in the boar reproductive tract and spermatozoa

Popis výsledku anglicky

Boar spermadhesins (AQN, AWN, PSP) are proteins containing a characteristic CUB domain, originally isolated from seminal plasma and ejaculated spermatozoa, with various role in reproduction. Unlike AQN and AWN spermadhesins, localization of porcine seminal plasma (PSP) proteins in boar reproductive tract has not been completely resolved. This work is focused on PSP protein localization in the boar reproductive organs and on spermatozoa. Using specific polyclonal antibodies, PSP I and PSP II proteins were immunodetected in tissue extracts from cauda epididymis, prostate, seminal vesicles and Cowper´s glands on the blots, and in secretory tissues of these organs by immunofluorescence. The ability of PSP proteins to bind the epididymal spermatozoa indicated their presence on cauda epididymal and ejaculated spermatozoa. PSP proteins are produced not only by seminal vesicles and prostate, but also by epididymis. However, their prospective role in sperm epididymal maturation is not clear

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Reproductive Immunology

ISSN

0165-0378

e-ISSN

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Svazek periodika

1

Číslo periodika v rámci svazku

78

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

9

Strana od-do

40-48

Kód UT WoS článku

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EID výsledku v databázi Scopus

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