Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins

Popis výsledku v původním jazyce

Recognition and binding of spermatozoa to the zona pellucida (ZP) is a crucial step in the fertilization process. This is the topic of many scientific groups, and so far many molecules on the sperm surface, involved in mentioned process, were identified.Sperm surface proteins of ejaculated and capacitated boar sperms were isolated by Pierce Cell Surface Isolation Kit and characterized by 1D, 2D electrophoreses, glycoprotein staining, hyaluronidase and proteolytic activities. Finally binding assay withZP was performed. Results have shown the quantitative and qualitative differences in both 1D and 2D electrophoretic profiles, glycoprotein profile was similar to 1D electrophoretic one. Neither hyaluronidase nor proteolytic activities were observed. Binding assay proved that interactions occur between both ejaculated and capacitated sperms. Protein interactions of capacitated sperm with ZP glycoproteins seem to be more specific.

Název v anglickém jazyce

Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins

Popis výsledku anglicky

Recognition and binding of spermatozoa to the zona pellucida (ZP) is a crucial step in the fertilization process. This is the topic of many scientific groups, and so far many molecules on the sperm surface, involved in mentioned process, were identified.Sperm surface proteins of ejaculated and capacitated boar sperms were isolated by Pierce Cell Surface Isolation Kit and characterized by 1D, 2D electrophoreses, glycoprotein staining, hyaluronidase and proteolytic activities. Finally binding assay withZP was performed. Results have shown the quantitative and qualitative differences in both 1D and 2D electrophoretic profiles, glycoprotein profile was similar to 1D electrophoretic one. Neither hyaluronidase nor proteolytic activities were observed. Binding assay proved that interactions occur between both ejaculated and capacitated sperms. Protein interactions of capacitated sperm with ZP glycoproteins seem to be more specific.

Druh

O - Ostatní výsledky

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů