Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F10%3A00346926" target="_blank" >RIV/86652036:_____/10:00346926 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices

Popis výsledku v původním jazyce

Over the last few years, targeted approaches have been complemented by profiling methods to assess possible unintended effects of transformation of GMO. Here we used Affymetrix microarray platform to evaluate transcriptional differences between commercial MON810 GM maize and non-transgenic crops. We took two common MON810/non-GM variety pairs and two farming practices. MON810 and comparable non-GM varieties have very low numbers of sequences with differential expression. We show, the differences betweena given MON810 variety and the non-GM do not depend on the assayed cultural conditions. Natural variation explained most of the variability in gene expression. Up to 37 % was dependent upon the variety and 32 % a result of the fertilization. The MON810had minor effect (9.7%) on gene expression. This indicates that transcriptional differences of conventionally-bred varieties and different environmental conditions should be taken into account in safety assessment studies of GM plants.

Název v anglickém jazyce

Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices

Popis výsledku anglicky

Over the last few years, targeted approaches have been complemented by profiling methods to assess possible unintended effects of transformation of GMO. Here we used Affymetrix microarray platform to evaluate transcriptional differences between commercial MON810 GM maize and non-transgenic crops. We took two common MON810/non-GM variety pairs and two farming practices. MON810 and comparable non-GM varieties have very low numbers of sequences with differential expression. We show, the differences betweena given MON810 variety and the non-GM do not depend on the assayed cultural conditions. Natural variation explained most of the variability in gene expression. Up to 37 % was dependent upon the variety and 32 % a result of the fertilization. The MON810had minor effect (9.7%) on gene expression. This indicates that transcriptional differences of conventionally-bred varieties and different environmental conditions should be taken into account in safety assessment studies of GM plants.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Molecular Biology

ISSN

0167-4412

e-ISSN

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Svazek periodika

73

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

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Kód UT WoS článku

000276911800009

EID výsledku v databázi Scopus

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