Asbestos exposure affects poly(ADP-ribose) polymerase-1 activity: role in asbestos-induced carcinogenesis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F11%3A00369504" target="_blank" >RIV/86652036:_____/11:00369504 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1093/mutage/ger020" target="_blank" >http://dx.doi.org/10.1093/mutage/ger020</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/mutage/ger020" target="_blank" >10.1093/mutage/ger020</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Asbestos exposure affects poly(ADP-ribose) polymerase-1 activity: role in asbestos-induced carcinogenesis
Popis výsledku v původním jazyce
Asbestos is known to induce malignant mesothelioma (MM). It is directly genotoxic by inducing DNA strand breaks. Poly(ADP-ribose) polymerase-1 (PARP1) is a protein with a function as a DNA damage sensor. To determine whether PARP1 is involved in asbestos-induced carcinogenesis, PARP1 expression and activity as well as DNA damage and repair were evaluated in circulating cells of asbestos-exposed subjects, MM patients and controls. Accumulation of the pre-mutagenic 8-hydroxy-2'-deoxyguanosine and elevatedPARP1 expression were found both in asbestos-exposed subjects and MM patients. Although PARP1 was highly expressed, its activity was relatively low in circulating cells as well as in MM biopsies. Low DNA repair efficiency was observed in lymphocytes from MM patients. To mimic PARP1 dysfunction, PARP1 expression and activity were induced in immortalised mesothelial cells by their exposure to asbestos in the presence of a PARP1 inhibitor, which resulted in transformation of the cells.
Název v anglickém jazyce
Asbestos exposure affects poly(ADP-ribose) polymerase-1 activity: role in asbestos-induced carcinogenesis
Popis výsledku anglicky
Asbestos is known to induce malignant mesothelioma (MM). It is directly genotoxic by inducing DNA strand breaks. Poly(ADP-ribose) polymerase-1 (PARP1) is a protein with a function as a DNA damage sensor. To determine whether PARP1 is involved in asbestos-induced carcinogenesis, PARP1 expression and activity as well as DNA damage and repair were evaluated in circulating cells of asbestos-exposed subjects, MM patients and controls. Accumulation of the pre-mutagenic 8-hydroxy-2'-deoxyguanosine and elevatedPARP1 expression were found both in asbestos-exposed subjects and MM patients. Although PARP1 was highly expressed, its activity was relatively low in circulating cells as well as in MM biopsies. Low DNA repair efficiency was observed in lymphocytes from MM patients. To mimic PARP1 dysfunction, PARP1 expression and activity were induced in immortalised mesothelial cells by their exposure to asbestos in the presence of a PARP1 inhibitor, which resulted in transformation of the cells.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
MUTAGENESIS
ISSN
0267-8357
e-ISSN
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Svazek periodika
26
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
7
Strana od-do
585-591
Kód UT WoS článku
000294554600002
EID výsledku v databázi Scopus
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