Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F11%3A00371924" target="_blank" >RIV/86652036:_____/11:00371924 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.reprotox.2011.09.005" target="_blank" >http://dx.doi.org/10.1016/j.reprotox.2011.09.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.reprotox.2011.09.005" target="_blank" >10.1016/j.reprotox.2011.09.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization

Popis výsledku v původním jazyce

Recognition of early determinants of disease onset has sparked an interest in paternally transmitted factors and their impact on the developing embryo. Acrylamide (AA),a widely distributed xenobiotic compound,is converted to its active metabolite glycidamide (GA) by the CYP2E1 enzyme.Based on its capacity to induce dominant lethal mutations,we hypothesized that paternal GA exposure would have a negative impact on embryonic genome activation,via GA-DNA and protamine adducts persisting in the fertilizingsperm. Using a combination of in vitro fertilization (IVF) techniques and RT-qPCR single embryo gene expression (SEGE), we studied the expression of key DNA repair genes and genes important for embryo development, at the 1-, 2-, 4- and 8-cell stage of the developing mouse embryo. Compared to controls paternal GA-exposure gave rise to an altered pattern of embryonic gene expression, with an initial reduced expression at early stages followed by increased expression at the 8-cell stage.

Název v anglickém jazyce

Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization

Popis výsledku anglicky

Recognition of early determinants of disease onset has sparked an interest in paternally transmitted factors and their impact on the developing embryo. Acrylamide (AA),a widely distributed xenobiotic compound,is converted to its active metabolite glycidamide (GA) by the CYP2E1 enzyme.Based on its capacity to induce dominant lethal mutations,we hypothesized that paternal GA exposure would have a negative impact on embryonic genome activation,via GA-DNA and protamine adducts persisting in the fertilizingsperm. Using a combination of in vitro fertilization (IVF) techniques and RT-qPCR single embryo gene expression (SEGE), we studied the expression of key DNA repair genes and genes important for embryo development, at the 1-, 2-, 4- and 8-cell stage of the developing mouse embryo. Compared to controls paternal GA-exposure gave rise to an altered pattern of embryonic gene expression, with an initial reduced expression at early stages followed by increased expression at the 8-cell stage.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA500520809" target="_blank" >IAA500520809: Mechanismy časné diferenciace embryonálních kmenových buněk</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reproductive Toxicology

ISSN

0890-6238

e-ISSN

—

Svazek periodika

32

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NO - Norské království

Počet stran výsledku

9

Strana od-do

463-471

Kód UT WoS článku

000297716900010

EID výsledku v databázi Scopus

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