Molecular mechanism for the selective impairment of cancer mitochondrial function by a mitochondrially targeted vitamin E analogue

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F12%3A00383182" target="_blank" >RIV/86652036:_____/12:00383182 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.bbabio.2012.05.005" target="_blank" >http://dx.doi.org/10.1016/j.bbabio.2012.05.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbabio.2012.05.005" target="_blank" >10.1016/j.bbabio.2012.05.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular mechanism for the selective impairment of cancer mitochondrial function by a mitochondrially targeted vitamin E analogue

Popis výsledku v původním jazyce

The effects of alpha-tocopheryl succinate (alpha-TOS), alpha-tocopheryl acetyl ether (alpha-TEA) and triphenylphosphonium-tagged vitamin E succinate (mitochondrially targeted vitamin E succinate; MitoVES) on energy-related mitochondrial functions were determined in mitochondria isolated from AS-30D hepatoma and rat liver, bovine heart sub-mitochondrial particles (SMPs), and in rodent and human carcinoma cell lines and rat hepatocytes. In isolated mitochondria, MitoVES stimulated basal respiration and ATP hydrolysis, but inhibited net state 3 (ADP-stimulated) respiration and Ca2+ uptake, by collapsing the membrane potential at low doses (1-10 mu M). Uncoupled mitochondrial respiration and basal respiration of SMPs were inhibited by the three drugs at concentrations at least one order of magnitude higher and with different efficacy: MitoVES> alpha-TEA>alpha-TOS. At high doses (>10 mu M), the respiratory complex II (CII) was the most sensitive MitoVES target. Acting as an uncoupler at low

Název v anglickém jazyce

Molecular mechanism for the selective impairment of cancer mitochondrial function by a mitochondrially targeted vitamin E analogue

Popis výsledku anglicky

The effects of alpha-tocopheryl succinate (alpha-TOS), alpha-tocopheryl acetyl ether (alpha-TEA) and triphenylphosphonium-tagged vitamin E succinate (mitochondrially targeted vitamin E succinate; MitoVES) on energy-related mitochondrial functions were determined in mitochondria isolated from AS-30D hepatoma and rat liver, bovine heart sub-mitochondrial particles (SMPs), and in rodent and human carcinoma cell lines and rat hepatocytes. In isolated mitochondria, MitoVES stimulated basal respiration and ATP hydrolysis, but inhibited net state 3 (ADP-stimulated) respiration and Ca2+ uptake, by collapsing the membrane potential at low doses (1-10 mu M). Uncoupled mitochondrial respiration and basal respiration of SMPs were inhibited by the three drugs at concentrations at least one order of magnitude higher and with different efficacy: MitoVES> alpha-TEA>alpha-TOS. At high doses (>10 mu M), the respiratory complex II (CII) was the most sensitive MitoVES target. Acting as an uncoupler at low

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP301%2F10%2F1937" target="_blank" >GAP301/10/1937: Mechanismy účinného zabíjení kmenových buněk rakoviny prsu</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica Et Biophysica Acta-Bioenergetics

ISSN

0005-2728

e-ISSN

—

Svazek periodika

1817

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

21

Strana od-do

1597-1607

Kód UT WoS článku

000306536400008

EID výsledku v databázi Scopus

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