The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F13%3A00396696" target="_blank" >RIV/86652036:_____/13:00396696 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1373/clinchem.2013.206375" target="_blank" >http://dx.doi.org/10.1373/clinchem.2013.206375</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1373/clinchem.2013.206375" target="_blank" >10.1373/clinchem.2013.206375</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

Popis výsledku v původním jazyce

There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acids, facilitating the measurement of small percentage differences and quantification of rare variants. dPCR may also be more reproducible and less susceptible to inhibition than quantitative real-time PCR (qPCR). Consequently, dPCR has the potential to have a substantial impact on research as well as diagnostic applications. However, as with qPCR, the ability to perform robust meaningful experiments requires careful design and adequate controls. To assist independent evaluation of experimental data, comprehensive disclosure of all relevant experimental details isrequired. To facilitate this process we present the Minimum Information for Publication of Quantitative Digital PCR Experiments guidelines. This report addresses known requirements for dPCR that have already been identified during this ea

Název v anglickém jazyce

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

Popis výsledku anglicky

There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acids, facilitating the measurement of small percentage differences and quantification of rare variants. dPCR may also be more reproducible and less susceptible to inhibition than quantitative real-time PCR (qPCR). Consequently, dPCR has the potential to have a substantial impact on research as well as diagnostic applications. However, as with qPCR, the ability to perform robust meaningful experiments requires careful design and adequate controls. To assist independent evaluation of experimental data, comprehensive disclosure of all relevant experimental details isrequired. To facilitate this process we present the Minimum Information for Publication of Quantitative Digital PCR Experiments guidelines. This report addresses known requirements for dPCR that have already been identified during this ea

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP303%2F10%2F1338" target="_blank" >GAP303/10/1338: Regulace buněčného objemu u gliových buněk v průběhu ischemie/reperfúze mozku</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinical Chemistry

ISSN

0009-9147

e-ISSN

—

Svazek periodika

59

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

892-902

Kód UT WoS článku

000321548500008

EID výsledku v databázi Scopus

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