Dye-Based High-Throughput qPCR in Microfluidic Platform BioMark

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F13%3A00427984" target="_blank" >RIV/86652036:_____/13:00427984 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1201/b14930-30" target="_blank" >http://dx.doi.org/10.1201/b14930-30</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1201/b14930-30" target="_blank" >10.1201/b14930-30</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dye-Based High-Throughput qPCR in Microfluidic Platform BioMark

Popis výsledku v původním jazyce

When using the dynamic array, each sample is mixed with each assay combinatorially in the integrated fluidic circuits resulting in 48 48 = 2304 or 96 96 = 9216 qPCR reactions. All the reactions are run in parallel under identical thermal and optical conditions; hence, no interplate calibration is needed and less bias is introduced. When the first BioMark dynamic array was launched in 2007 hydrolysis probes were used exclusively for gene expression analysis, but the advantages of dsDNA binding dye basedanalysis are in some cases essential: primer design and assay validation process is less complex, in many academic cases the recent primer libraries can be used directly, melt profiles of PCR products can be recorded after each run, and gene expression profiling experiments costs are lower. We developed a temperature protocol for BioMark, which reflects the specific attributes of its optical system together with high background fluorescence of the dsDNA binding dyes in room temperature.

Název v anglickém jazyce

Dye-Based High-Throughput qPCR in Microfluidic Platform BioMark

Popis výsledku anglicky

When using the dynamic array, each sample is mixed with each assay combinatorially in the integrated fluidic circuits resulting in 48 48 = 2304 or 96 96 = 9216 qPCR reactions. All the reactions are run in parallel under identical thermal and optical conditions; hence, no interplate calibration is needed and less bias is introduced. When the first BioMark dynamic array was launched in 2007 hydrolysis probes were used exclusively for gene expression analysis, but the advantages of dsDNA binding dye basedanalysis are in some cases essential: primer design and assay validation process is less complex, in many academic cases the recent primer libraries can be used directly, melt profiles of PCR products can be recorded after each run, and gene expression profiling experiments costs are lower. We developed a temperature protocol for BioMark, which reflects the specific attributes of its optical system together with high background fluorescence of the dsDNA binding dyes in room temperature.

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

PCR Technology

ISBN

9781439848050

Počet stran výsledku

17

Strana od-do

323-339

Počet stran knihy

457

Název nakladatele

CRC Press Inc

Místo vydání

Washington

Kód UT WoS kapitoly

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