Progress of sperm Izumo1 relocation during spontaneous acrosome reaction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F14%3A00421166" target="_blank" >RIV/86652036:_____/14:00421166 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/14:10209859

Výsledek na webu

<a href="http://dx.doi.org/10.1530/REP-13-0193" target="_blank" >http://dx.doi.org/10.1530/REP-13-0193</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1530/REP-13-0193" target="_blank" >10.1530/REP-13-0193</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Progress of sperm Izumo1 relocation during spontaneous acrosome reaction

Popis výsledku v původním jazyce

It has been recently shown in mice that sperm undergo acrosome reaction (AR) by passing through cumulus cells, furthermore the acrosome-reacted sperm can bind to zona pellucida, and consequently fertilize the egg. During AR, the relocation of the primaryfusion protein Izumo1 into the equatorial segment is crucial for sperm-egg fusion. There is a high rate of spontaneous AR in rodents, with up to 60% in promiscuous species. The aim of this study was to clarify whether the Izumo1 relocation in sperm after spontaneous and induced AR is the same; and whether there is a correlation between the speed of Izumo1 relocation and species-specific mating behaviour in field mice. Immunofluorescent detection of Izumo1 dynamics during the in vitro capacitation, spontaneous, calcium ionophore and progesterone induced AR was monitored. Our results show that during spontaneous AR there is a clear Izumo1 relocation from the acrosomal cap to the equatorial segment, and further over the whole sperm head.

Název v anglickém jazyce

Progress of sperm Izumo1 relocation during spontaneous acrosome reaction

Popis výsledku anglicky

It has been recently shown in mice that sperm undergo acrosome reaction (AR) by passing through cumulus cells, furthermore the acrosome-reacted sperm can bind to zona pellucida, and consequently fertilize the egg. During AR, the relocation of the primaryfusion protein Izumo1 into the equatorial segment is crucial for sperm-egg fusion. There is a high rate of spontaneous AR in rodents, with up to 60% in promiscuous species. The aim of this study was to clarify whether the Izumo1 relocation in sperm after spontaneous and induced AR is the same; and whether there is a correlation between the speed of Izumo1 relocation and species-specific mating behaviour in field mice. Immunofluorescent detection of Izumo1 dynamics during the in vitro capacitation, spontaneous, calcium ionophore and progesterone induced AR was monitored. Our results show that during spontaneous AR there is a clear Izumo1 relocation from the acrosomal cap to the equatorial segment, and further over the whole sperm head.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reproduction

ISSN

1470-1626

e-ISSN

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Svazek periodika

147

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

231-240

Kód UT WoS článku

000330557700012

EID výsledku v databázi Scopus

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