Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F14%3A00427908" target="_blank" >RIV/86652036:_____/14:00427908 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/14:00432511

Výsledek na webu

<a href="http://dx.doi.org/10.1155/2014/138350" target="_blank" >http://dx.doi.org/10.1155/2014/138350</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2014/138350" target="_blank" >10.1155/2014/138350</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture

Popis výsledku v původním jazyce

This study aimed to provide a molecular signature for enriched adult human stem/progenitor spermatogonia during short-term (<2 weeks) and long-term culture (up to more than 14 months) in comparison to human testicular fibroblasts and human embryonic stemcells. Human spermatogonia were isolated by CD49f magnetic activated cell sorting and collagen(-)/laminin(+) matrix binding from primary testis cultures obtained from ten adult men. For transcriptomic analysis, single spermatogonia-like cells were collected based on their morphology and dimensions using a micromanipulation system from the enriched germ cell cultures. Immunocytochemical, RT-PCR and microarray analyses revealed that the analyzed populations of cells were distinct at the molecular level.The germ-and pluripotency-associated genes and genes of differentiation/spermatogenesis pathway were highly expressed in enriched short-term cultured spermatogonia. After long-term culture, a proportion of cells retained and aggravated th

Název v anglickém jazyce

Differential Gene Expression Profiling of Enriched Human Spermatogonia after Short- and Long-Term Culture

Popis výsledku anglicky

This study aimed to provide a molecular signature for enriched adult human stem/progenitor spermatogonia during short-term (<2 weeks) and long-term culture (up to more than 14 months) in comparison to human testicular fibroblasts and human embryonic stemcells. Human spermatogonia were isolated by CD49f magnetic activated cell sorting and collagen(-)/laminin(+) matrix binding from primary testis cultures obtained from ten adult men. For transcriptomic analysis, single spermatogonia-like cells were collected based on their morphology and dimensions using a micromanipulation system from the enriched germ cell cultures. Immunocytochemical, RT-PCR and microarray analyses revealed that the analyzed populations of cells were distinct at the molecular level.The germ-and pluripotency-associated genes and genes of differentiation/spermatogenesis pathway were highly expressed in enriched short-term cultured spermatogonia. After long-term culture, a proportion of cells retained and aggravated th

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BioMed Research International

ISSN

2314-6133

e-ISSN

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Svazek periodika

2014

Číslo periodika v rámci svazku

138350

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

17

Strana od-do

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Kód UT WoS článku

000333307400001

EID výsledku v databázi Scopus

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