Local dynamics of proteins and DNA evaluated from crystallographic B factors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F14%3A00437245" target="_blank" >RIV/86652036:_____/14:00437245 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1107/S1399004714014631" target="_blank" >http://dx.doi.org/10.1107/S1399004714014631</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1107/S1399004714014631" target="_blank" >10.1107/S1399004714014631</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Local dynamics of proteins and DNA evaluated from crystallographic B factors

Popis výsledku v původním jazyce

The dynamics of protein and nucleic acid structures is as important as their average static picture. The local molecular dynamics concealed in diffraction images is expressed as so-called B factors. To find out how the crystal-derived B factors representthe dynamic behaviour of atoms and residues of proteins and DNA in their complexes, the distributions of scaled B factors from a carefully curated data set of over 700 protein-DNA crystal structures were analyzed [Schneider et al. (2014), Nucleic AcidsRes. 42, 3381-3394].Amino acids and nucleotides were categorized based on their molecular neighbourhood as solvent-accessible, solvent-inaccessible (i.e. forming the protein core) or lying at protein-protein or protein-DNA interfaces; the backbone and side-chain atoms were analyzed separately.The B factors of two types of crystal-ordered water molecules were also analyzed.The analysis confirmed several expected features of protein and DNA dynamics,but also revealed surprising facts.Solve

Název v anglickém jazyce

Local dynamics of proteins and DNA evaluated from crystallographic B factors

Popis výsledku anglicky

The dynamics of protein and nucleic acid structures is as important as their average static picture. The local molecular dynamics concealed in diffraction images is expressed as so-called B factors. To find out how the crystal-derived B factors representthe dynamic behaviour of atoms and residues of proteins and DNA in their complexes, the distributions of scaled B factors from a carefully curated data set of over 700 protein-DNA crystal structures were analyzed [Schneider et al. (2014), Nucleic AcidsRes. 42, 3381-3394].Amino acids and nucleotides were categorized based on their molecular neighbourhood as solvent-accessible, solvent-inaccessible (i.e. forming the protein core) or lying at protein-protein or protein-DNA interfaces; the backbone and side-chain atoms were analyzed separately.The B factors of two types of crystal-ordered water molecules were also analyzed.The analysis confirmed several expected features of protein and DNA dynamics,but also revealed surprising facts.Solve

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Crystallographica Section D-Biological Crystallography

ISSN

0907-4449

e-ISSN

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Svazek periodika

70

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

DK - Dánské království

Počet stran výsledku

7

Strana od-do

2413-2419

Kód UT WoS článku

000341819500016

EID výsledku v databázi Scopus

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