Selenium supplementation induces mitochondrial biogenesis in trophoblasts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F15%3A00450924" target="_blank" >RIV/86652036:_____/15:00450924 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.placenta.2015.06.010" target="_blank" >http://dx.doi.org/10.1016/j.placenta.2015.06.010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.placenta.2015.06.010" target="_blank" >10.1016/j.placenta.2015.06.010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Selenium supplementation induces mitochondrial biogenesis in trophoblasts

Popis výsledku v původním jazyce

Introduction: Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium. Methods: Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-l alpha and NRF-1 was examined by western blotting. Results: Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells.

Název v anglickém jazyce

Selenium supplementation induces mitochondrial biogenesis in trophoblasts

Popis výsledku anglicky

Introduction: Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium. Methods: Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-l alpha and NRF-1 was examined by western blotting. Results: Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Placenta

ISSN

0143-4004

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

363-369

Kód UT WoS článku

000359875000012

EID výsledku v databázi Scopus

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