The effect of mitochondrially targeted anticancer agents on mitochondrial (super)complexes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F15%3A00522123" target="_blank" >RIV/86652036:_____/15:00522123 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/protocol/10.1007%2F978-1-4939-2288-8_15" target="_blank" >https://link.springer.com/protocol/10.1007%2F978-1-4939-2288-8_15</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-2288-8_15" target="_blank" >10.1007/978-1-4939-2288-8_15</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of mitochondrially targeted anticancer agents on mitochondrial (super)complexes

Popis výsledku v původním jazyce

The mitochondrial respiratory chain is organized into dynamic high molecular weight complexes that associate to form supercomplexes. The function of these SCs is to minimize the production of reactive oxygen species (ROS) generated during electron transfer within them and to efficiently transfer electrons to complex IV. These supra-molecular structures as well as whole mitochondria are stress-responsive and respond to mitochondrially targeted anti-cancer agent by destabilization and induction of massive production of ROS leading to apoptosis. We have recently developed mitochondrially targeted anti-cancer agents epitomized by the mitochondrially targeted analogue of the redox-silent compound vitamin E succinate, which belongs to the group of agents that kill cancer cells via their mitochondria-destabilizing activity, referred to as mitocans. To understand the molecular mechanism of the effect of such agents, the use of native blue gel electrophoresis and clear native electrophoresis coupled with in-gel activity assays, are methods of choice. The relevant methodology is described in this chapter.

Název v anglickém jazyce

The effect of mitochondrially targeted anticancer agents on mitochondrial (super)complexes

Popis výsledku anglicky

The mitochondrial respiratory chain is organized into dynamic high molecular weight complexes that associate to form supercomplexes. The function of these SCs is to minimize the production of reactive oxygen species (ROS) generated during electron transfer within them and to efficiently transfer electrons to complex IV. These supra-molecular structures as well as whole mitochondria are stress-responsive and respond to mitochondrially targeted anti-cancer agent by destabilization and induction of massive production of ROS leading to apoptosis. We have recently developed mitochondrially targeted anti-cancer agents epitomized by the mitochondrially targeted analogue of the redox-silent compound vitamin E succinate, which belongs to the group of agents that kill cancer cells via their mitochondria-destabilizing activity, referred to as mitocans. To understand the molecular mechanism of the effect of such agents, the use of native blue gel electrophoresis and clear native electrophoresis coupled with in-gel activity assays, are methods of choice. The relevant methodology is described in this chapter.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Methods in molecular biology

ISSN

1940-6029

e-ISSN

—

Svazek periodika

1265

Číslo periodika v rámci svazku

2015

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

195-208

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-84958608088