Kinetic Model of the Action of 17 alpha-Ethynylestradiol on the Capacitation of Mouse Sperm, Monitored by HPLC-MS/MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F20%3A00541327" target="_blank" >RIV/86652036:_____/20:00541327 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/20:10410333 RIV/00064165:_____/20:10410333

Výsledek na webu

<a href="https://www.mdpi.com/2073-4344/10/1/124" target="_blank" >https://www.mdpi.com/2073-4344/10/1/124</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/catal10010124" target="_blank" >10.3390/catal10010124</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Kinetic Model of the Action of 17 alpha-Ethynylestradiol on the Capacitation of Mouse Sperm, Monitored by HPLC-MS/MS

Popis výsledku v původním jazyce

17 alpha-Ethynylestradiol (EE2), a synthetic estrogen used in contraceptive pills, is resistant to hepatic degradation and is excreted in the urine. It is chemically stable and has a negative impact on the endocrine system. The aim of this work was to mathematically describe the possible interaction of EE2 (200, 20, and 2 mu g/L) with sperm estrogen receptors during sperm maturation, which is called capacitation. The concentrations of the unbound EE2 remaining in capacitating medium during 180 min of sperm capacitation were determined at 30 min intervals by high performance liquid chromatography with tandem mass spectrometric detection (HPLC-MS/MS) and the data obtained (relative concentrations B-t) were subjected to kinetic analysis. The suggested kinetic schema was described by the system of differential equations with the optimization of rate constants used to calculate the theoretical B-t values. Optimal parameters (overall rate constants K-1-K-5 and molar ratio n) were determined by searching the minimum of absolute values of the difference between theoretical and experimental B-t values. These values were used for the design of the theoretical B(t) curves which fit to experimental points. The proposed kinetic model assumes the formation of an unstable adduct between EE2 and the receptor in cytoplasm, which acts as an autocatalytic agent and gradually decomposes.

Název v anglickém jazyce

Kinetic Model of the Action of 17 alpha-Ethynylestradiol on the Capacitation of Mouse Sperm, Monitored by HPLC-MS/MS

Popis výsledku anglicky

17 alpha-Ethynylestradiol (EE2), a synthetic estrogen used in contraceptive pills, is resistant to hepatic degradation and is excreted in the urine. It is chemically stable and has a negative impact on the endocrine system. The aim of this work was to mathematically describe the possible interaction of EE2 (200, 20, and 2 mu g/L) with sperm estrogen receptors during sperm maturation, which is called capacitation. The concentrations of the unbound EE2 remaining in capacitating medium during 180 min of sperm capacitation were determined at 30 min intervals by high performance liquid chromatography with tandem mass spectrometric detection (HPLC-MS/MS) and the data obtained (relative concentrations B-t) were subjected to kinetic analysis. The suggested kinetic schema was described by the system of differential equations with the optimization of rate constants used to calculate the theoretical B-t values. Optimal parameters (overall rate constants K-1-K-5 and molar ratio n) were determined by searching the minimum of absolute values of the difference between theoretical and experimental B-t values. These values were used for the design of the theoretical B(t) curves which fit to experimental points. The proposed kinetic model assumes the formation of an unstable adduct between EE2 and the receptor in cytoplasm, which acts as an autocatalytic agent and gradually decomposes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Catalysts

ISSN

2073-4344

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

124

Kód UT WoS článku

000516825000124

EID výsledku v databázi Scopus

2-s2.0-85078474738