Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F24%3A00583677" target="_blank" >RIV/86652036:_____/24:00583677 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/24:10482788 RIV/00216208:11310/24:10482788 RIV/00023001:_____/24:00084682

Výsledek na webu

<a href="https://www.spandidos-publications.com/10.3892/br.2024.1738" target="_blank" >https://www.spandidos-publications.com/10.3892/br.2024.1738</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/br.2024.1738" target="_blank" >10.3892/br.2024.1738</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases

Popis výsledku v původním jazyce

The current standard for graft rejection surveillance is endomyocardial biopsy (EMB), an invasive procedure with rare but potentially serious complications. Detection of circulating donor-derived cell-free DNA (ddcfDNA) is an option for noninvasive monitoring of graft injury and rejection. A 63-year-old man and a 65-year-old woman were monitored by EMB for allograft rejection. A total of 48 single-nucleotide polymorphisms with a minor allele frequency range of 0.4-0.5 were screened to distinguish donor and recipient DNA based on homozygosity, and digital droplet PCR was used to analyze ddcfDNA concentrations. Both subjects suffered rejection within the first 6 months after transplantation. The maximal ddcfDNA level of 270 copies (cp)/ml during EMB-confirmed acute cellular rejection (ACR, mild grade 1R/2, patient 1), and the maximal concentration of 1,846 cp/ml in the case of EMB-confirmed antibody-mediated rejection (AMR, grade 1+, patient 2), was detected. Individual monitoring of ddcfDNA dynamics from the 1st to the 6th month posttransplant reflected cardiac graft injury in patients suffering ACR or AMR, meaning that ddcfDNA may serve as a noninvasive biomarker.

Název v anglickém jazyce

Monitoring of plasma circulating donor DNA reflects cardiac graft injury: Report of two cases

Popis výsledku anglicky

The current standard for graft rejection surveillance is endomyocardial biopsy (EMB), an invasive procedure with rare but potentially serious complications. Detection of circulating donor-derived cell-free DNA (ddcfDNA) is an option for noninvasive monitoring of graft injury and rejection. A 63-year-old man and a 65-year-old woman were monitored by EMB for allograft rejection. A total of 48 single-nucleotide polymorphisms with a minor allele frequency range of 0.4-0.5 were screened to distinguish donor and recipient DNA based on homozygosity, and digital droplet PCR was used to analyze ddcfDNA concentrations. Both subjects suffered rejection within the first 6 months after transplantation. The maximal ddcfDNA level of 270 copies (cp)/ml during EMB-confirmed acute cellular rejection (ACR, mild grade 1R/2, patient 1), and the maximal concentration of 1,846 cp/ml in the case of EMB-confirmed antibody-mediated rejection (AMR, grade 1+, patient 2), was detected. Individual monitoring of ddcfDNA dynamics from the 1st to the 6th month posttransplant reflected cardiac graft injury in patients suffering ACR or AMR, meaning that ddcfDNA may serve as a noninvasive biomarker.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/NU20-06-00061" target="_blank" >NU20-06-00061: Možnosti využití volně cirkulující gDNA a mtDNA v diagnostice rejekce štěpu po transplantaci srdce.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical Reports

ISSN

2049-9434

e-ISSN

2049-9442

Svazek periodika

20

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

7

Strana od-do

50

Kód UT WoS článku

001162521300001

EID výsledku v databázi Scopus

2-s2.0-85184431316