MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F24%3A00616698" target="_blank" >RIV/86652036:_____/24:00616698 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1534580723006512?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1534580723006512?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.devcel.2023.12.001" target="_blank" >10.1016/j.devcel.2023.12.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement

Popis výsledku v původním jazyce

Microtubule doublets (MTDs) comprise an incomplete microtubule (Btubule) attached to the side of a complete cylindrical microtubule. These compound microtubules are conserved in cilia across the tree of life, however, the mechanisms by which MTDs form and are maintained in vivo remain poorly understood. Here, we identify microtubule-associated protein 9 (MAP9) as an MTD-associated protein. We demonstrate that C. elegans MAPH-9, a MAP9 homolog, is present during MTD assembly and localizes exclusively to MTDs, a preference that is in part mediated by tubulin polyglutamylation. We find that loss of MAPH-9 causes ultrastructural MTD defects, including shortened and/or squashed Btubules with reduced numbers of protofilaments, dysregulated axonemal motor velocity, and perturbed cilia function. Because we find that the mammalian ortholog MAP9 localizes to axonemes in cultured mammalian cells and mouse tissues, we propose that MAP9/MAPH-9 plays a conserved role in regulating ciliary motors and supporting the structure of axonemal MTDs.

Název v anglickém jazyce

MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement

Popis výsledku anglicky

Microtubule doublets (MTDs) comprise an incomplete microtubule (Btubule) attached to the side of a complete cylindrical microtubule. These compound microtubules are conserved in cilia across the tree of life, however, the mechanisms by which MTDs form and are maintained in vivo remain poorly understood. Here, we identify microtubule-associated protein 9 (MAP9) as an MTD-associated protein. We demonstrate that C. elegans MAPH-9, a MAP9 homolog, is present during MTD assembly and localizes exclusively to MTDs, a preference that is in part mediated by tubulin polyglutamylation. We find that loss of MAPH-9 causes ultrastructural MTD defects, including shortened and/or squashed Btubules with reduced numbers of protofilaments, dysregulated axonemal motor velocity, and perturbed cilia function. Because we find that the mammalian ortholog MAP9 localizes to axonemes in cultured mammalian cells and mouse tissues, we propose that MAP9/MAPH-9 plays a conserved role in regulating ciliary motors and supporting the structure of axonemal MTDs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

<a href="/cs/project/GX19-27477X" target="_blank" >GX19-27477X: Cytoskeletální mechanika axonálního navádění</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Developmental Cell

ISSN

1534-5807

e-ISSN

1878-1551

Svazek periodika

59

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

24

Strana od-do

—

Kód UT WoS článku

001177666300001

EID výsledku v databázi Scopus

2-s2.0-85182334372