Automatic Image-Based Method for Quantitative Analysis of Photosynthetic Cell Cultures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F16%3A00460374" target="_blank" >RIV/86652079:_____/16:00460374 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28140/16:43874682

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-3-319-32034-2_34" target="_blank" >http://dx.doi.org/10.1007/978-3-319-32034-2_34</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-319-32034-2_34" target="_blank" >10.1007/978-3-319-32034-2_34</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Automatic Image-Based Method for Quantitative Analysis of Photosynthetic Cell Cultures

Popis výsledku v původním jazyce

This work deals with an automatic quantitative analysis of photosynthetic cell cultures. It uses images captured by a confocal fluorescent microscope for automatic determination the number of cells in sample containing complex 3D structure of cell clusters. Experiments were performed on the confocal microscope Leica TCS SP8 X. The cell nuclei were stained by SYBR Green fluorescent DNA binding marker. In the first step we used combination of adaptive thresholding to found out areas where nuclei were located. Proposed segmentation steps allowed reduction of noise and artefacts. Z-axis position was obtained as a location of peak from intensity profile. Finally model of scene can be created by emplacement of spheres with adequate diameter to found 3D coordinates. Number of cells per volumetric unit were determined in structurally different culture samples of cell cultures Chenopodium rubrum (Cr) and Solanum lycopersicum (To). The results were verified by manual counting

Název v anglickém jazyce

Automatic Image-Based Method for Quantitative Analysis of Photosynthetic Cell Cultures

Popis výsledku anglicky

This work deals with an automatic quantitative analysis of photosynthetic cell cultures. It uses images captured by a confocal fluorescent microscope for automatic determination the number of cells in sample containing complex 3D structure of cell clusters. Experiments were performed on the confocal microscope Leica TCS SP8 X. The cell nuclei were stained by SYBR Green fluorescent DNA binding marker. In the first step we used combination of adaptive thresholding to found out areas where nuclei were located. Proposed segmentation steps allowed reduction of noise and artefacts. Z-axis position was obtained as a location of peak from intensity profile. Finally model of scene can be created by emplacement of spheres with adequate diameter to found 3D coordinates. Number of cells per volumetric unit were determined in structurally different culture samples of cell cultures Chenopodium rubrum (Cr) and Solanum lycopersicum (To). The results were verified by manual counting

Druh

D - Stať ve sborníku

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

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Projekt

<a href="/cs/project/LO1415" target="_blank" >LO1415: CzechGlobe 2020 - Rozvoj Centra pro studium dopadů globální změny klimatu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Hybrid Artificial Intelligent Systems: 11th International Conference, HAIS 2016, Seville, Spain, April 18-20, 2016, Proceedings

ISBN

978-3-319-32033-5

ISSN

0302-9743

e-ISSN

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Počet stran výsledku

12

Strana od-do

402-103

Název nakladatele

Springer International Publishing

Místo vydání

Cham

Místo konání akce

Seville

Datum konání akce

18. 4. 2016

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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