Determination of the activity signature of key carbohydrate metabolism enzymes in phenolic-rich grapevine tissues
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F16%3A00467450" target="_blank" >RIV/86652079:_____/16:00467450 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.17344/acsi.2016.2484" target="_blank" >http://dx.doi.org/10.17344/acsi.2016.2484</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17344/acsi.2016.2484" target="_blank" >10.17344/acsi.2016.2484</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of the activity signature of key carbohydrate metabolism enzymes in phenolic-rich grapevine tissues
Popis výsledku v původním jazyce
Physiological studies in plants often require enzyme extraction from tissues containing high concentrations of phenols and polyphenols. Unless removed or neutralized, such compounds may hinder extraction, inactivate enzymes, and interfere with enzyme detection. The following protocol for activity assays for enzymes of primary carbohydrate metabolism, while based on our recently published one for quantitative measurement of activities using coupled spectrophotometric assays in a 96-well format, is tailored to the complexities of phenolic- and anthocyanin-rich extracts from grapevine leaf. As a case study we applied the protocol to grapevine leaf samples infected with plant pathogenic bacteria 'Candidatus Phytoplasma solani', known to alter carbohydrate metabolism in grapevine. The described adaptations may be useful for determination of metabolic fingerprints for physiological phenotyping of other plant species with inherently high levels of phenolic compounds. nn
Název v anglickém jazyce
Determination of the activity signature of key carbohydrate metabolism enzymes in phenolic-rich grapevine tissues
Popis výsledku anglicky
Physiological studies in plants often require enzyme extraction from tissues containing high concentrations of phenols and polyphenols. Unless removed or neutralized, such compounds may hinder extraction, inactivate enzymes, and interfere with enzyme detection. The following protocol for activity assays for enzymes of primary carbohydrate metabolism, while based on our recently published one for quantitative measurement of activities using coupled spectrophotometric assays in a 96-well format, is tailored to the complexities of phenolic- and anthocyanin-rich extracts from grapevine leaf. As a case study we applied the protocol to grapevine leaf samples infected with plant pathogenic bacteria 'Candidatus Phytoplasma solani', known to alter carbohydrate metabolism in grapevine. The described adaptations may be useful for determination of metabolic fingerprints for physiological phenotyping of other plant species with inherently high levels of phenolic compounds. nn
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EH - Ekologie – společenstva
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/LO1415" target="_blank" >LO1415: CzechGlobe 2020 - Rozvoj Centra pro studium dopadů globální změny klimatu</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Acta Chimica Slovenica
ISSN
1318-0207
e-ISSN
—
Svazek periodika
63
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
SI - Slovinská republika
Počet stran výsledku
6
Strana od-do
757-762
Kód UT WoS článku
000392743500009
EID výsledku v databázi Scopus
2-s2.0-85003875024