Two-dimensional Root Phenotyping System Based on Root Growth on Black Filter Paper and Recirculation Micro-irrigation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F16%3A00468570" target="_blank" >RIV/86652079:_____/16:00468570 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/16:43910846

Výsledek na webu

<a href="http://dx.doi.org/10.17221/121/2015-CJGPB" target="_blank" >http://dx.doi.org/10.17221/121/2015-CJGPB</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/121/2015-CJGPB" target="_blank" >10.17221/121/2015-CJGPB</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Two-dimensional Root Phenotyping System Based on Root Growth on Black Filter Paper and Recirculation Micro-irrigation

Popis výsledku v původním jazyce

Plant root system architecture (RSA) has an important role in crop production, particularly for water and nutrient uptake under limiting conditions. In the last few years, several root phenotyping methods have been developed. Here we present a new technique which has been developed for non-destructive, inexpensive and high-throughput root growth studies and RSA analyses. To illustrate the potential applications, this method was tested in an experiment with nitrogen and phosphorous deficiencies in a nutrient solution, affecting RSA parameters of two spring barley varieties (Bojos and Barke). This technique is based on root growth on vertically positioned black filter paper (30 60 cm) placed between two black plastic (PVC-P) foils and micro-irrigation systems providing the recirculation of nutrient solution. The pre-germinated seeds were placed in the slit between two plastic bars which carry the filter paper and plastic sheets and fix the plant in the vertical position. This system allows easy repeated non-invasive access to roots for their measuring and sampling. Eighteen days after transplanting the root imaging was done using an RGB digital camera. To evaluate the root architecture parameters the “SmartRoot software was used. The results revealed that the system is able to detect changes in RSA which are caused mainly by P deficiency (particularly changes in lateral root length and total root area). It can be concluded that this technique has a great potential for non-destructive root growth studies, RSA measurement and root sampling. © 2016, Czech Academy of Agricultural Sciences.

Název v anglickém jazyce

Two-dimensional Root Phenotyping System Based on Root Growth on Black Filter Paper and Recirculation Micro-irrigation

Popis výsledku anglicky

Plant root system architecture (RSA) has an important role in crop production, particularly for water and nutrient uptake under limiting conditions. In the last few years, several root phenotyping methods have been developed. Here we present a new technique which has been developed for non-destructive, inexpensive and high-throughput root growth studies and RSA analyses. To illustrate the potential applications, this method was tested in an experiment with nitrogen and phosphorous deficiencies in a nutrient solution, affecting RSA parameters of two spring barley varieties (Bojos and Barke). This technique is based on root growth on vertically positioned black filter paper (30 60 cm) placed between two black plastic (PVC-P) foils and micro-irrigation systems providing the recirculation of nutrient solution. The pre-germinated seeds were placed in the slit between two plastic bars which carry the filter paper and plastic sheets and fix the plant in the vertical position. This system allows easy repeated non-invasive access to roots for their measuring and sampling. Eighteen days after transplanting the root imaging was done using an RGB digital camera. To evaluate the root architecture parameters the “SmartRoot software was used. The results revealed that the system is able to detect changes in RSA which are caused mainly by P deficiency (particularly changes in lateral root length and total root area). It can be concluded that this technique has a great potential for non-destructive root growth studies, RSA measurement and root sampling. © 2016, Czech Academy of Agricultural Sciences.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

—

Projekt

<a href="/cs/project/LO1415" target="_blank" >LO1415: CzechGlobe 2020 - Rozvoj Centra pro studium dopadů globální změny klimatu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Genetics and Plant Breeding

ISSN

1212-1975

e-ISSN

—

Svazek periodika

52

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

64-70

Kód UT WoS článku

000378158200003

EID výsledku v databázi Scopus

2-s2.0-84975138430