Isolation and Screening of Lipase Producing Microorganisms from Natural Sources

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F17%3A00473780" target="_blank" >RIV/86652079:_____/17:00473780 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation and Screening of Lipase Producing Microorganisms from Natural Sources

Popis výsledku v původním jazyce

Lipases are the enzymes that bring about a wide range of bioconversion reactions. Lipases occur widely in nature, but microbial lipases are commercially most significant. Lipase catalyzedtrans-esterification, hydrolysis and esterification are the important class of reactions for technological applications in fats and oil industry, dairy industry, pharmaceuticals and bakery industry. The main reason for the steadily growing interest in lipases is also because of their enantio-selective, regioselective and chemo-selective nature. Enzyme-mediated reactions are attractive alternatives to tedious and expensive chemical methods. Present investigation aimed at bio-prospection of lipase producing microbial isolates from various natural sources. The better enzyme producing microbial isolate for its ability to produce the enzyme was investigated under diverged nutrient conditions of lipase assay. A total of twenty two microbial isolates were isolated from different sources, out of which nine fungal isolates and thirteen bacterial isolates were found to show appreciable lipase producing ability. These cultures were further used to find out their quantitative production of lipase in liquid media with time. It was observed that in majority of the cases, the lipase production increased with time, reaching the maximum on fifth day and decreasing thereafter.

Název v anglickém jazyce

Isolation and Screening of Lipase Producing Microorganisms from Natural Sources

Popis výsledku anglicky

Lipases are the enzymes that bring about a wide range of bioconversion reactions. Lipases occur widely in nature, but microbial lipases are commercially most significant. Lipase catalyzedtrans-esterification, hydrolysis and esterification are the important class of reactions for technological applications in fats and oil industry, dairy industry, pharmaceuticals and bakery industry. The main reason for the steadily growing interest in lipases is also because of their enantio-selective, regioselective and chemo-selective nature. Enzyme-mediated reactions are attractive alternatives to tedious and expensive chemical methods. Present investigation aimed at bio-prospection of lipase producing microbial isolates from various natural sources. The better enzyme producing microbial isolate for its ability to produce the enzyme was investigated under diverged nutrient conditions of lipase assay. A total of twenty two microbial isolates were isolated from different sources, out of which nine fungal isolates and thirteen bacterial isolates were found to show appreciable lipase producing ability. These cultures were further used to find out their quantitative production of lipase in liquid media with time. It was observed that in majority of the cases, the lipase production increased with time, reaching the maximum on fifth day and decreasing thereafter.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

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OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Indian Journal of Ecology

ISSN

0304-5250

e-ISSN

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Svazek periodika

44

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

IN - Indická republika

Počet stran výsledku

5

Strana od-do

19-23

Kód UT WoS článku

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EID výsledku v databázi Scopus

2-s2.0-85024918630