A correlative approach, combining chlorophyll a fluorescence, reflectance, and Raman spectroscopy, for monitoring hydration induced changes in Antarctic lichen Dermatocarpon polyphyllizum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F19%3A00495975" target="_blank" >RIV/86652079:_____/19:00495975 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/19:00112324

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S1386142518308886?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1386142518308886?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.saa.2018.09.036" target="_blank" >10.1016/j.saa.2018.09.036</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A correlative approach, combining chlorophyll a fluorescence, reflectance, and Raman spectroscopy, for monitoring hydration induced changes in Antarctic lichen Dermatocarpon polyphyllizum

Popis výsledku v původním jazyce

Lichens are successful colonizers in extreme environments worldwide, and they are considered to have played an important role during the evolution of life. Here, we have used a correlative approach, combining three optical signals (chlorophyll a fluorescence (ChlF), reflectance, and Raman spectra), to monitor hydration induced changes in photosynthetic properties of an Antarctic chlorolichen Dermatocarpon polyphyllizum. We measured these three signals from this lichen at different stages (after 4 h, 24 h, and 48 h) of hydration, and compared the data obtained from this lichen in “dry state” as well as in different “hydrated state”. We found that dry state of this lichen has: (1) no variable ChlF, (2) high reflectance, with no red-edge and almost zero photochemical reflectance index (PRI), and (3) low-intensity Raman bands of their carotenoids. Furthermore, 4 h of hydration, increased its relative water content (RWC) by 93%, showed red-edge in reflectance spectra, and changed the maximum quantum yield of PSII photochemistry (Fv/Fm) from 0 to 0.57 ± 0.01. We found that reflectance indices, normalized difference index (NDVI) and PRI, significantly differed between brown and black/green surface areas, at all hydration stages, whereas, a shift in the Raman ν1(C[dbnd]C) band, between brown and black/green surface areas, occurred in 24 h or 48 h hydrated samples. These data indicate that hydration shortly (within 4 h) activated functions of photosynthetic apparatus, and the de novo synthesis of carotenoids occured in 24 h or 48 h. Furthermore, exposure to high irradiance (2000 μmol photons m−2s−1), in 48 h hydrated lichen, significantly reduced Fv/Fm(signifies photoinhibition) and increased PRI (represents changes in xanthophyll pigments). We conclude that the implication of such a correlative approach is highly useful for understanding survival and protective mechanisms on extremophile photosynthetic organisms.

Název v anglickém jazyce

A correlative approach, combining chlorophyll a fluorescence, reflectance, and Raman spectroscopy, for monitoring hydration induced changes in Antarctic lichen Dermatocarpon polyphyllizum

Popis výsledku anglicky

Lichens are successful colonizers in extreme environments worldwide, and they are considered to have played an important role during the evolution of life. Here, we have used a correlative approach, combining three optical signals (chlorophyll a fluorescence (ChlF), reflectance, and Raman spectra), to monitor hydration induced changes in photosynthetic properties of an Antarctic chlorolichen Dermatocarpon polyphyllizum. We measured these three signals from this lichen at different stages (after 4 h, 24 h, and 48 h) of hydration, and compared the data obtained from this lichen in “dry state” as well as in different “hydrated state”. We found that dry state of this lichen has: (1) no variable ChlF, (2) high reflectance, with no red-edge and almost zero photochemical reflectance index (PRI), and (3) low-intensity Raman bands of their carotenoids. Furthermore, 4 h of hydration, increased its relative water content (RWC) by 93%, showed red-edge in reflectance spectra, and changed the maximum quantum yield of PSII photochemistry (Fv/Fm) from 0 to 0.57 ± 0.01. We found that reflectance indices, normalized difference index (NDVI) and PRI, significantly differed between brown and black/green surface areas, at all hydration stages, whereas, a shift in the Raman ν1(C[dbnd]C) band, between brown and black/green surface areas, occurred in 24 h or 48 h hydrated samples. These data indicate that hydration shortly (within 4 h) activated functions of photosynthetic apparatus, and the de novo synthesis of carotenoids occured in 24 h or 48 h. Furthermore, exposure to high irradiance (2000 μmol photons m−2s−1), in 48 h hydrated lichen, significantly reduced Fv/Fm(signifies photoinhibition) and increased PRI (represents changes in xanthophyll pigments). We conclude that the implication of such a correlative approach is highly useful for understanding survival and protective mechanisms on extremophile photosynthetic organisms.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

ISSN

1386-1425

e-ISSN

—

Svazek periodika

208

Číslo periodika v rámci svazku

feb

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

13-23

Kód UT WoS článku

000449900700003

EID výsledku v databázi Scopus

2-s2.0-85054147877