Lignans Extract from Knotwood of Norway Spruce-A Possible New Weapon against GTDs
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F22%3A00557269" target="_blank" >RIV/86652079:_____/22:00557269 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62156489:43510/22:43921475
Výsledek na webu
<a href="https://www.researchgate.net/publication/359587875_Lignans_Extract_from_Knotwood_of_Norway_Spruce-A_Possible_New_Weapon_against_GTDs" target="_blank" >https://www.researchgate.net/publication/359587875_Lignans_Extract_from_Knotwood_of_Norway_Spruce-A_Possible_New_Weapon_against_GTDs</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/jof8040357" target="_blank" >10.3390/jof8040357</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Lignans Extract from Knotwood of Norway Spruce-A Possible New Weapon against GTDs
Popis výsledku v původním jazyce
Grapevine trunk diseases (GTDs) pose a major threat to the wine industry worldwide. Currently, efficient biological methods or chemical compounds are not available for the treatment of infected grapevines. In the present study, we used an extract from the knotwood of spruce trees as a biological control against GTDs. Our in vitro trial was focused on the antifungal effects of the extract against the most common GTD pathogens-Cadophora luteo-olivacea, Dactylonectria torresensis, Diaporthe ampelina, Diaporthe bohemiae, Diplodia seriata, Eutypa lata, and Phaeoacremonium minimum. Our in vitro trial revealed a high antifungal effect of the extract against all tested fungi. The inhibition rates varied among the different species from 30% to 100% using 1 mg center dot mL(-1) extract. Subsequently, the efficiency of the extract was supported by an in planta experiment. Commercial grafts of Vitis vinifera were treated with the extract and planted. The total genomic DNA of grapevines was extracted 10 days and 180 days after the treatment. The fungal microbial diversities of the treated/untreated plants were compared using high-throughput amplicon sequencing (HTAS). Treated plants showed 76.9% lower relative abundance of the genus Diaporthe and 70% lower relative abundance of the genus Phaeoacremonium 10 days after treatment. A similar scenario was observed for the genus Cadophora 180 days after treatment, where treated plants showed 76% lower relative abundance of this genus compared with untreated grapevines.
Název v anglickém jazyce
Lignans Extract from Knotwood of Norway Spruce-A Possible New Weapon against GTDs
Popis výsledku anglicky
Grapevine trunk diseases (GTDs) pose a major threat to the wine industry worldwide. Currently, efficient biological methods or chemical compounds are not available for the treatment of infected grapevines. In the present study, we used an extract from the knotwood of spruce trees as a biological control against GTDs. Our in vitro trial was focused on the antifungal effects of the extract against the most common GTD pathogens-Cadophora luteo-olivacea, Dactylonectria torresensis, Diaporthe ampelina, Diaporthe bohemiae, Diplodia seriata, Eutypa lata, and Phaeoacremonium minimum. Our in vitro trial revealed a high antifungal effect of the extract against all tested fungi. The inhibition rates varied among the different species from 30% to 100% using 1 mg center dot mL(-1) extract. Subsequently, the efficiency of the extract was supported by an in planta experiment. Commercial grafts of Vitis vinifera were treated with the extract and planted. The total genomic DNA of grapevines was extracted 10 days and 180 days after the treatment. The fungal microbial diversities of the treated/untreated plants were compared using high-throughput amplicon sequencing (HTAS). Treated plants showed 76.9% lower relative abundance of the genus Diaporthe and 70% lower relative abundance of the genus Phaeoacremonium 10 days after treatment. A similar scenario was observed for the genus Cadophora 180 days after treatment, where treated plants showed 76% lower relative abundance of this genus compared with untreated grapevines.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
<a href="/cs/project/EF19_073%2F0016670" target="_blank" >EF19_073/0016670: Interní grantová schémata Mendelovy univerzity v Brně</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Fungi
ISSN
2309-608X
e-ISSN
2309-608X
Svazek periodika
8
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
19
Strana od-do
357
Kód UT WoS článku
000786993100001
EID výsledku v databázi Scopus
2-s2.0-85128369544