Phenotyping drought tolerance and yield performance of barley using a combination of imaging methods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F23%3A00571782" target="_blank" >RIV/86652079:_____/23:00571782 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/23:43923266 RIV/60646594:_____/23:N0000003

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0098847223001090?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0098847223001090?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.envexpbot.2023.105314" target="_blank" >10.1016/j.envexpbot.2023.105314</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phenotyping drought tolerance and yield performance of barley using a combination of imaging methods

Popis výsledku v původním jazyce

Drought resistance represents a complex of traits that are differently employed depending on drought type, severity and timing. Hence, a relatively comprehensive assessment of morphological and physiological pheno-typic response to drought is required. We evaluated the dynamic responses of six barley genotypes, representing a wide range of drought tolerance, to continuous drying and re-watering using non-invasively measured pa-rameters based on red-green-blue (RGB), thermal infrared and chlorophyll fluorescence imaging within an automated phenotyping platform. We identified three critical points in drought progress: i) 50% level of avail-able soil water, ii) wilting point, iii) full plant recovery after re-watering. However, the individual monitored parameters showed the potential to evaluate drought sensitivity at different points since the onset of drying. The correlation with relative yield response gradually increased for the side projected leaf area (SPA) and reached the maximum at the point of full recovery. The actual quantum yield of photosystem II (phi PSII) showed the highest correlation with a relative grain yield around wilting point. In contrast, the relative leaf temperature difference demonstrated a high correlation with yield response earlier, at 50% of available water. The highest correlations with the relative yield response were obtained for the colour RGB analysis at the wilting point and after recovery, particularly for khaki, beige, dark-green and olive-green colours. Multiple regression with parameters providing Pearson's correlation coefficient R > 0.5 slightly improved the estimation of relative yield response to drought but ensured significant improvement of absolute grain yield estimation under drought stress. This study shows that combining the phenotyping methods representing different morphological and physiological traits allows not only the assessment of drought tolerance (based on relative yield response to drought) which is crucial for selecting the genetic resources for the subsequent breeding process but also allows to test the yield performance of new genotypes under drought stress.

Název v anglickém jazyce

Phenotyping drought tolerance and yield performance of barley using a combination of imaging methods

Popis výsledku anglicky

Drought resistance represents a complex of traits that are differently employed depending on drought type, severity and timing. Hence, a relatively comprehensive assessment of morphological and physiological pheno-typic response to drought is required. We evaluated the dynamic responses of six barley genotypes, representing a wide range of drought tolerance, to continuous drying and re-watering using non-invasively measured pa-rameters based on red-green-blue (RGB), thermal infrared and chlorophyll fluorescence imaging within an automated phenotyping platform. We identified three critical points in drought progress: i) 50% level of avail-able soil water, ii) wilting point, iii) full plant recovery after re-watering. However, the individual monitored parameters showed the potential to evaluate drought sensitivity at different points since the onset of drying. The correlation with relative yield response gradually increased for the side projected leaf area (SPA) and reached the maximum at the point of full recovery. The actual quantum yield of photosystem II (phi PSII) showed the highest correlation with a relative grain yield around wilting point. In contrast, the relative leaf temperature difference demonstrated a high correlation with yield response earlier, at 50% of available water. The highest correlations with the relative yield response were obtained for the colour RGB analysis at the wilting point and after recovery, particularly for khaki, beige, dark-green and olive-green colours. Multiple regression with parameters providing Pearson's correlation coefficient R > 0.5 slightly improved the estimation of relative yield response to drought but ensured significant improvement of absolute grain yield estimation under drought stress. This study shows that combining the phenotyping methods representing different morphological and physiological traits allows not only the assessment of drought tolerance (based on relative yield response to drought) which is crucial for selecting the genetic resources for the subsequent breeding process but also allows to test the yield performance of new genotypes under drought stress.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental and Experimental Botany

ISSN

0098-8472

e-ISSN

1873-7307

Svazek periodika

209

Číslo periodika v rámci svazku

MAY

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

105314

Kód UT WoS článku

000966712700001

EID výsledku v databázi Scopus

2-s2.0-85151019389