Characterizing isoprene production in cyanobacteria-Insights into the effects of light, temperature, and isoprene on Synechocystis sp. PCC 6803

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F23%3A00572613" target="_blank" >RIV/86652079:_____/23:00572613 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/23:00572613

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0960852423004947?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0960852423004947?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biortech.2023.129068" target="_blank" >10.1016/j.biortech.2023.129068</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterizing isoprene production in cyanobacteria-Insights into the effects of light, temperature, and isoprene on Synechocystis sp. PCC 6803

Popis výsledku v původním jazyce

Engineering cyanobacteria for the production of isoprene and other terpenoids has gained increasing attention in the field of biotechnology. Several studies have addressed optimization of isoprene synthesis in cyanobacteria via enzyme and pathway engineering. However, only little attention has been paid to the optimization of cultivation conditions. In this study, an isoprene-producing strain of Synechocystis sp. PCC 6803 and two control strains were grown under a variety of cultivation conditions. Isoprene production, as quantified by modified membrane inlet mass spectrometer (MIMS) and interpreted using Flux Balance Analysis (FBA), increased under violet light and at elevated temperature. Increase of thermotolerance in the isoprene producer was attributed to the physical presence of isoprene, similar to plants. The results demonstrate a beneficial effect of isoprene on cell survival at higher temperatures. This increased thermotolerance opens new possibilities for sustainable bio-production of isoprene and other products.

Název v anglickém jazyce

Characterizing isoprene production in cyanobacteria-Insights into the effects of light, temperature, and isoprene on Synechocystis sp. PCC 6803

Popis výsledku anglicky

Engineering cyanobacteria for the production of isoprene and other terpenoids has gained increasing attention in the field of biotechnology. Several studies have addressed optimization of isoprene synthesis in cyanobacteria via enzyme and pathway engineering. However, only little attention has been paid to the optimization of cultivation conditions. In this study, an isoprene-producing strain of Synechocystis sp. PCC 6803 and two control strains were grown under a variety of cultivation conditions. Isoprene production, as quantified by modified membrane inlet mass spectrometer (MIMS) and interpreted using Flux Balance Analysis (FBA), increased under violet light and at elevated temperature. Increase of thermotolerance in the isoprene producer was attributed to the physical presence of isoprene, similar to plants. The results demonstrate a beneficial effect of isoprene on cell survival at higher temperatures. This increased thermotolerance opens new possibilities for sustainable bio-production of isoprene and other products.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/EF16_026%2F0008413" target="_blank" >EF16_026/0008413: Strategické partnerství pro environmentální technologie a produkci energie</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bioresource Technology

ISSN

0960-8524

e-ISSN

1873-2976

Svazek periodika

380

Číslo periodika v rámci svazku

JUL 2023

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

129068

Kód UT WoS článku

000991095500001

EID výsledku v databázi Scopus

2-s2.0-85153498734