Ammonia oxidation and nitrate reduction marker genes are key indicators of nitrogen losses in temperate forest catchments

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652079%3A_____%2F23%3A00580054" target="_blank" >RIV/86652079:_____/23:00580054 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00025798:_____/23:10168735 RIV/60076658:12310/23:43906656

Výsledek na webu

<a href="https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.16441" target="_blank" >https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.16441</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1462-2920.16441" target="_blank" >10.1111/1462-2920.16441</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ammonia oxidation and nitrate reduction marker genes are key indicators of nitrogen losses in temperate forest catchments

Popis výsledku v původním jazyce

Chronic nitrogen inputs can alleviate N limitation and potentially impose N losses in forests, indicated by soil enrichment in N-15 over N-14. However, the complexity of the nitrogen cycle hinders accurate quantification of N fluxes. Simultaneously, soil ecologists are striving to find meaningful indicators to characterise the openness of the nitrogen cycle. We integrate soil delta N-15 with constrained ecosystem N losses and the functional gene potential of the soil microbiome in 14 temperate forest catchments. We show that N losses are associated with soil delta N-15 and that delta N-15 scales with the abundance of soil bacteria. The abundance of the archaeal amoA gene, representing the first step in nitrification (ammonia oxidation to nitrite), followed by the abundance of narG and napA genes, associated with the first step in denitrification (nitrate reduction to nitrite), explains most of the variability in soil delta N-15. These genes are more informative than the denitrification genes nirS and nirK, which are directly linked to N2O production. Nitrite formation thus appears to be the critical step associated with N losses. Furthermore, we show that the genetic potential for ammonia oxidation and nitrate reduction is representative of forest soil N-15 enrichment and thus indicative of ecosystem N losses.

Název v anglickém jazyce

Ammonia oxidation and nitrate reduction marker genes are key indicators of nitrogen losses in temperate forest catchments

Popis výsledku anglicky

Chronic nitrogen inputs can alleviate N limitation and potentially impose N losses in forests, indicated by soil enrichment in N-15 over N-14. However, the complexity of the nitrogen cycle hinders accurate quantification of N fluxes. Simultaneously, soil ecologists are striving to find meaningful indicators to characterise the openness of the nitrogen cycle. We integrate soil delta N-15 with constrained ecosystem N losses and the functional gene potential of the soil microbiome in 14 temperate forest catchments. We show that N losses are associated with soil delta N-15 and that delta N-15 scales with the abundance of soil bacteria. The abundance of the archaeal amoA gene, representing the first step in nitrification (ammonia oxidation to nitrite), followed by the abundance of narG and napA genes, associated with the first step in denitrification (nitrate reduction to nitrite), explains most of the variability in soil delta N-15. These genes are more informative than the denitrification genes nirS and nirK, which are directly linked to N2O production. Nitrite formation thus appears to be the critical step associated with N losses. Furthermore, we show that the genetic potential for ammonia oxidation and nitrate reduction is representative of forest soil N-15 enrichment and thus indicative of ecosystem N losses.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40104 - Soil science

Projekt

<a href="/cs/project/GA20-19471S" target="_blank" >GA20-19471S: GeoMicLink: Vliv mikrobiální komunity na retenci živin v povodích</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Microbiology

ISSN

1462-2912

e-ISSN

1462-2920

Svazek periodika

25

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

2049-2053

Kód UT WoS článku

001002386100001

EID výsledku v databázi Scopus

2-s2.0-85161577925