Ice crystallization under cryogenic cooling in lipid membrane nanoconfined geometry: Time-resolved structural dynamics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2FCZ______%3A_____%2F23%3AN0000011" target="_blank" >RIV/CZ______:_____/23:N0000011 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/CZ______:_____/23:N0000089 RIV/00216208:11320/23:10474547

Výsledek na webu

<a href="https://doi.org/10.1016/j.jcis.2022.12.095" target="_blank" >https://doi.org/10.1016/j.jcis.2022.12.095</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jcis.2022.12.095" target="_blank" >10.1016/j.jcis.2022.12.095</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ice crystallization under cryogenic cooling in lipid membrane nanoconfined geometry: Time-resolved structural dynamics

Popis výsledku v původním jazyce

Time-resolved structural investigations of crystallization of water in lipid/protein/salt mesophases at cryogenic temperatures are significant for comprehension of ice nanocrystal nucleation kinetics in lipid membranous systems and can lead to a better understanding of how to experimentally retard the ice for-mation that obstructs the protein crystal structure determination. Here, we present a time-resolved syn-chrotron microfocus X-ray diffraction (TR-XRD) study based on 40,000 frames that revealed the dynamics of water-to-ice crystallization in a lipid/protein/salt mesophase subjected to cryostream cool -ing at 100 K. The monoolein/hemoglobin/salt/water system was chosen as a model composition related to protein-loaded lipid cubic phases (LCP) broadly used for the crystallization of proteins. Under confine-ment in the nanoscale geometry, metastable short-living cubic ice (Ic) rapidly crystallized well before the formation of hexagonal ice (Ih). The detected early nanocrystalline states of water-to-ice transformation in multicomponent systems are relevant to a broad spectrum of technologies and understanding of nat-ural phenomena, including crystallization, physics of water nanoconfinement, and rational design of anti-freezing and cryopreservation systems.(c) 2022 Elsevier Inc. All rights reserved.

Název v anglickém jazyce

Ice crystallization under cryogenic cooling in lipid membrane nanoconfined geometry: Time-resolved structural dynamics

Popis výsledku anglicky

Time-resolved structural investigations of crystallization of water in lipid/protein/salt mesophases at cryogenic temperatures are significant for comprehension of ice nanocrystal nucleation kinetics in lipid membranous systems and can lead to a better understanding of how to experimentally retard the ice for-mation that obstructs the protein crystal structure determination. Here, we present a time-resolved syn-chrotron microfocus X-ray diffraction (TR-XRD) study based on 40,000 frames that revealed the dynamics of water-to-ice crystallization in a lipid/protein/salt mesophase subjected to cryostream cool -ing at 100 K. The monoolein/hemoglobin/salt/water system was chosen as a model composition related to protein-loaded lipid cubic phases (LCP) broadly used for the crystallization of proteins. Under confine-ment in the nanoscale geometry, metastable short-living cubic ice (Ic) rapidly crystallized well before the formation of hexagonal ice (Ih). The detected early nanocrystalline states of water-to-ice transformation in multicomponent systems are relevant to a broad spectrum of technologies and understanding of nat-ural phenomena, including crystallization, physics of water nanoconfinement, and rational design of anti-freezing and cryopreservation systems.(c) 2022 Elsevier Inc. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10308 - Astronomy (including astrophysics,space science)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Colloid and Interface Science

ISSN

0021-9797

e-ISSN

1095-7103

Svazek periodika

634

Číslo periodika v rámci svazku

february

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

757-768

Kód UT WoS článku

000909869800001

EID výsledku v databázi Scopus

2-s2.0-85144480406