Design and Fabrication of 3D printed In-situ Crystallization Plates for Probing Microcrystals in External Electric Field

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2FCZ______%3A_____%2F24%3AN0000020" target="_blank" >RIV/CZ______:_____/24:N0000020 - isvavai.cz</a>

Výsledek na webu

<a href="https://journals.iucr.org/j/issues/2024/03/00/te5134/index.html" target="_blank" >https://journals.iucr.org/j/issues/2024/03/00/te5134/index.html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1107/S1600576724002140" target="_blank" >10.1107/S1600576724002140</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Design and Fabrication of 3D printed In-situ Crystallization Plates for Probing Microcrystals in External Electric Field

Popis výsledku v původním jazyce

X-ray crystallography is an established tool to probe the structure of macromolecules with atomic resolution. Compared with alternative techniques such as single-particle cryo-electron microscopy and micro-electron diffraction, X-ray crystallography is uniquely suited to room-temperature studies and for obtaining a detailed picture of macromolecules subjected to an external electric field (EEF). The impact of an EEF on proteins has been extensively explored through single-crystal X-ray crystallography, which works well with larger high-quality protein crystals. This article introduces a novel design for a 3D-printed in situ crystallization plate that serves a dual purpose: fostering crystal growth and allowing the concurrent examination of the effects of an EEF on crystals of varying sizes. The plate's compatibility with established X-ray crystallography techniques is evaluated.

Název v anglickém jazyce

Design and Fabrication of 3D printed In-situ Crystallization Plates for Probing Microcrystals in External Electric Field

Popis výsledku anglicky

X-ray crystallography is an established tool to probe the structure of macromolecules with atomic resolution. Compared with alternative techniques such as single-particle cryo-electron microscopy and micro-electron diffraction, X-ray crystallography is uniquely suited to room-temperature studies and for obtaining a detailed picture of macromolecules subjected to an external electric field (EEF). The impact of an EEF on proteins has been extensively explored through single-crystal X-ray crystallography, which works well with larger high-quality protein crystals. This article introduces a novel design for a 3D-printed in situ crystallization plate that serves a dual purpose: fostering crystal growth and allowing the concurrent examination of the effects of an EEF on crystals of varying sizes. The plate's compatibility with established X-ray crystallography techniques is evaluated.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

<a href="/cs/project/EF16_019%2F0000789" target="_blank" >EF16_019/0000789: Pokročilý výzkum s využitím fotonů a částic vytvořených vysoce intenzivními lasery</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Crystallography

ISSN

1600-5767

e-ISSN

—

Svazek periodika

57

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

842-847

Kód UT WoS článku

001256619300024

EID výsledku v databázi Scopus

2-s2.0-85195407155