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15 581 (0,109s)

Projekt

Novel protease inhibitors utilisable against human and animal patogens, and in plant protection (IBS5007316)

Proposed project is based on the discovery of an unusually small and fairly active Kazal-type proteinase inhibitor GmSP12 that occurs in the silk of a pyralid moth Galleria mellonella. It is planed to prepare several modifications of this inhibitor i...

EB - Genetika a molekulární biologie

  • 2003 - 2005
  • 2 675 tis. Kč
  • 1 700 tis. Kč
  • AV ČR
Projekt

Large-scale implementation of an optimum technology for high-productivity processes with recombinant methylotrophic yeasts (1P05OE189)

innovative stabile process of recombinant yeasts Pichia pastoris cultivation, stabile technology of cultivation, production of recombinant protein......

EI - Biotechnologie a bionika

  • 2005 - 2007
  • 4 645 tis. Kč
  • 2 776 tis. Kč
  • MŠMT
Projekt

Enzymatic synthesis of chitooligomers for sustainable zero pesticide agriculture (LUC23097)

. Heterologous expression of new chitinase from Aspergillus oryzae (AoChit) in yeast expression system Pichia pastoris, production optimization and purification of AoChit. Heterologous expression

Biochemistry and molecular biology

  • 2023 - 2026
  • 5 688 tis. Kč
  • 5 688 tis. Kč
  • MŠMT
Projekt

Characterization and application of fungal non-heme oxidases (LD12049)

will be constructed. Selected enzymes will be expressed in heterologous hosts, purified...

EI - Biotechnologie a bionika

  • 2012 - 2015
  • 1 741 tis. Kč
  • 1 741 tis. Kč
  • MŠMT
Projekt

Study of the role of different isoenzymes of aspartic proteases in the virulence of Candida spp. (GA303/04/0432)

functions of Sap 9, 10, and vacuolar protease, we will express these enzymes in Pichia pastoris and E. coli. These proteases will be isolated and characterized......

CE - Biochemie

  • 2004 - 2006
  • 1 215 tis. Kč
  • 1 215 tis. Kč
  • GA ČR
Projekt

Expression of genes involved in the osmotolerance of non-conventional yeasts in Saccharomyces cerevisiae (GA204/98/0475)

The cells response to osmotic stress depends on glycerol metabolism and its regulation. Genomic DNA libraries from osmotolerant yeasts Pichia sorbitophila and Zygosaccharomyces rouxii will be used to isolate genes whose products are involved...

EB - Genetika a molekulární biologie

  • 1998 - 2000
  • 1 577 tis. Kč
  • 1 229 tis. Kč
  • GA ČR
Projekt

Multiple tick legumains in blood processing, innate immunity and pathogen transmission (GA14-33693S)

RNAi experiments. Selected IrAEs will be recombinantly expressed in P. pastoris expressional profiling experiments over tick stages and tissues demonstrated that IrAEs are mainly expressed in tick guts and hemocyt...

GJ - Choroby a škůdci zvířat, veterinární medicina

  • 2014 - 2016
  • 4 994 tis. Kč
  • 4 994 tis. Kč
  • GA ČR
Projekt

Preparation of recombinant plant virus coat protein in bacteria and yeast (GA522/99/0437)

We plane to prepare the coat protein gene of the model quarantine strawberry vein banding caulimovirus (SVBV), introduce this gene in appropriate content to vectors and produce it as recombinant protein in E.coli bacteria and in the methylotrophic ye...

CE - Biochemie

  • 1999 - 2001
  • 2 936 tis. Kč
  • 2 404 tis. Kč
  • GA ČR
Projekt

Biosimilars building of knowledge base for production of therapeutic proteins using heterologous expression systems. (2A-2TP1/030)

Proteins produced in heterologous expression systems, are the fastest growing and detailed utilisation of individual expression systems, yeast and more advanced will be exploited in the project. The outputs of the ...

EI - Biotechnologie a bionika

  • 2007 - 2011
  • 23 738 tis. Kč
  • 17 666 tis. Kč
  • MPO
Projekt

Construction of the New Eukaryotic Expression System (1QS600220554)

, high-yield, inducible protein expression system capable of producing post for the development of a tightly inducible protein expression system. The suitability of the developed system for structural and ...

EB - Genetika a molekulární biologie

  • 2005 - 2008
  • 1 514 tis. Kč
  • 1 514 tis. Kč
  • AV ČR
  • 1 - 10 z 15 581