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24 718 (0,115s)

Výsledek výzkumu

Isolation PCR-ready DNA from samples containing PCR inhibitors

Magnetic nonporous hydrophilic P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres containing carboxyl groups were used for purification of DNA isolated from the lyophilisates of four strains of Trichophyton fungi (without cult...

EB - Genetika a molekulární biologie

  • 2007
  • D
Výsledek výzkumu

Functionalized magnetic microspheres with hydrophilic properties for molecular diagnostic applications

Magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres ? P(HEMA-co-EDMA), P(HEMA-co-GMA) and PGMA ? were prepared by dispersion polymerisation in the presence of iron oxide nanoparticles....

CD - Makromolekulární chemie

  • 2009
  • Jx
Výsledek výzkumu

Hydrophilic particles designed for PCR applications

Magnetic hydrophilic nonporous P(HEMA-co-EDMA), P(HEMA-coGMA) and PGMA microspheres were prepared by dispersion (co)polymerization of HEMA and EDMA or GMA in the presence of several kinds of magnetite.It was found that some...

EE - Mikrobiologie, virologie

  • 2003
  • D
Výsledek výzkumu

Functionalised magnetic microsphereswith hydrophilic properties for molecular diagnostic applications

Magnetic nonporous hydrophilic microspheres poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate)-(P(HEMA-co-EDMA), poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-P(HEMA-co-GMA), and poly(glycidyl methacr...

EB - Genetika a molekulární biologie

  • 2009
  • Jx
Výsledek výzkumu

Magnetic hydrophilic methacrylate-based polymer microspheres designed for polymerase chain reaction applications

Magnetic hydrophilic nonporous P(HEMA-co-EDMA), P(HEMA-co-GMA) and PGMA in the preparation of magnetic carriers interfered with PCR. DNA isolated from bacterial cells of Bifidobacterium longum was used in PCR evalu...

EE - Mikrobiologie, virologie

  • 2004
  • Jx
Výsledek výzkumu

Magnetic Microspheres for DNA Diagnostics and DNA Delivery

Magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) [P(HEMA-EDMA))], poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [P(HEMA-GMA)], and poly(glycidyl methacrylate) [PGMA] m...

EB - Genetika a molekulární biologie

  • 2010
  • O
Výsledek výzkumu

Hydrophilic magnetic non-porous microspheres for molecular diagnostics applications

. Magnetic nonporous hydrophilic microspheres poly(2-hydroxyethyl methacrylate-coThe following applications of magnetic particles in analytical chemistry and molecular diagnostics are dominant: separation or purifi...

EI - Biotechnologie a bionika

  • 2013
  • O
Výsledek výzkumu

Hydrophilic magnetic nonporous microspheres for bacterial DNA isolation

Polymerase chain reaction (PCR)- based methods are specific and sensitive tools for idnetification of microorganisms. The occurence of false negative results is a great problem in the analysis of real samples. A rapid, small-scale DNA isolation metho...

EB - Genetika a molekulární biologie

  • 2009
  • Jx
Výsledek výzkumu

Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.

Non-selective isolation of high-quality DNA can be performed using carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres. This proc...

EB - Genetika a molekulární biologie

  • 2010
  • O
Výsledek výzkumu

Magnetic hydrophilic methacrylate-based polymer microspheres designed for PCR-ready DNA isolation

Newly designed magnetic nonporous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) - P(HEMA-co-EDMA), poly(glycidyl methacrylate) - PGMA and P(HEMA-co-GMA) microspheres carrying carboxyl groups were used for isolation of...

EE - Mikrobiologie, virologie

  • 2004
  • D
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