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Výsledek výzkumu

Knockout of MITOGEN-ACTIVATED PROTEIN KINASE 3 causes barley root resistance against Fusarium graminearum

suggest that TALEN-based knockout of HvMPK3 leads to barley root resistance against activator-like effector nuclease (TALEN)-based knockout lines of barley (Hordeum vulgare L.) MAPK 3 (HvMPK3 KO)...

Plant sciences, botany

  • 2022
  • Jimp
  • Odkaz
Výsledek výzkumu

TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley

differential proteomic analysis we show that TALEN-induced MPK3 knock-out lines of barley (HvMPK3 KO) exhibit constitutive downregulation of defense related proteins such as PR that the same protein families were less prone to flg2...

Biochemistry and molecular biology

  • 2021
  • Jimp
  • Odkaz
Výsledek výzkumu

Efficient disruption of endogenous Bombyx gene by TAL effector nucleases

TALENS are engineered nucleases used for targeted gene disruptions in various organisms. We examined the potential of using full length TALENs for site-specific mutagenesis in silkworm. Three full-length TALEN pairs were sy...

EB - Genetika a molekulární biologie

  • 2013
  • Jx
  • Odkaz
Výsledek výzkumu

Efficient TALEN construction for Bombyx mori gene targeting

to the induction of mutations at target sites. We showed earlier that ZFNs and TALENs are able protocol, we modified one of the reported truncated TALEN scaffolds and optimized suitable for the preselection of highly efficient ...

EB - Genetika a molekulární biologie

  • 2013
  • Jx
  • Odkaz
Výsledek výzkumu

The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitly

Transcription activator-like effector nucleases (TALENs) are custom-made homologous recombination requiring homologous DNA as a template for the repair. TALENs insects. We will describe here a simple TALEN-based mutagenesis...

EB - Genetika a molekulární biologie

  • 2014
  • Jx
  • Odkaz
Výsledek výzkumu

Mitochondrial phosphoenolpyruvate carboxylase contributes to carbon fixation in the diatom Phaeodactylum tricornutum at low inorganic carbon concentrations

generated and analyzed individual knockout cell lines of PEPC1 and PEPC2, as well as a PEPC1/2 double-knockout strain. While we could not detect an altered phenotype in the PEPC1 knockout strains at ambient, low or high DI...

Cell biology

  • 2022
  • Jimp
  • Odkaz
Výsledek výzkumu

Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases

(TALENs). We employed TALEN-assisted HR in zygotes to introduce constructs encoding TALENs exhibit high targeting efficiency superior to that of zinc-finger nucleases assay toassess TALENs activity and specificity...

EB - Genetika a molekulární biologie

  • 2014
  • Jx
  • Odkaz
Výsledek výzkumu

The use of TALENs for linseed transformation

This work describes the creation and the use of vector constructs containg TALENs (transcription activator-like effector nucleases) for the transformation of flax (Linum usitatissimum L.) in order to modify the fatty acid content. The use of...

GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

  • 2021
  • Jost
  • Odkaz
Výsledek výzkumu

Targeted modulation of alternative splicing by TALE-directed chromatin editing

To reveal how local chromatin modulates the splicing outcome we developed a new method based on Transcription Activator-Like Effector Nuclease (TALEN) technology. We replaced the nuclease domain with chromatin modifying domain and tested how...

EB - Genetika a molekulární biologie

  • 2015
  • O
Výsledek výzkumu

Adaptation to excess acetylcholine by downregulation of adrenoceptors and muscarinic receptors in lungs of acetylcholinesterase knockout mice

acetylcholinesterase knockout mice...

ED - Fyziologie

  • 2007
  • Jx
  • 1 - 10 z 476