Prediction of gestational diabetes on the basis of steroid metabolism
Project goals
Gestational diabetes mellitus (GDM) is a frequent complication. We have previously investigated the role of candidate genes and their genetic variability in the pathophysiology of GDM. The obtained data and current knowledge showing an expression of numerous isoforms of steroid sensitive ion channels in pancreas demonstrate their role in the regulation of insulin secretion and insulin sensitivity. An extensive rise of steroid levels in pregnancy prompted us to address a question how the bioactive steroids in maternal and mixed umbilical blood correlate with glucose tolerance, how the activities of steroidogenic enzymes are linked to glucose metabolism and how the aforementioned data change with gestational age. We intend to suggest prediction models for assessment of GDM risk based on steroid levels in blood of pregnant and non-pregnant women as well as possible modifications of GDM treatment.
Keywords
biomarkersgestační diabetesmetabolomikametabolomicsGestational diabetesbiomarkerypredikcepredictionpatofyziologieneuroaktivní steroidyenzymy steroidogenezypankreaspathophysiologyneuroactive steroidssteroidogenic enzymespancreas
Public support
Provider
Ministry of Health
Programme
Programme to support medical applied research in 2015 to 2022
Call for proposals
Zdravotnický AV 3 (SMZ0201701)
Main participants
Endokrinologický ústav
Contest type
VS - Public tender
Contract ID
17-30528A
Alternative language
Project name in Czech
Predikce gestačního diabetu na základě metabolizmu steroidů
Annotation in Czech
Gestační diabetes mellitus (GDM) je častou komplikací. V minulosti jsme se zabývali úlohou kandidátních genů a jejich genetickou variabilitou v patofyziologii GDM. Získané údaje a poznatky v literatuře uvádějící expresi četných izoforem forem steroid-senzitivních iontových kanálů v pankreatu dokazují jejich úlohu při regulaci inzulínové sekrece a inzulínové senzitivity. Výrazné zvýšení steroidních hladin v těhotenství nás vedlo k otázkám, jak bioaktivní steroidy v mateřské a ve smíšené pupečníkové krvi korelují s glukózovou tolerancí, jak jsou aktivity enzymů steroidogenezy spojeny s glukózovým metabolismem a jak se uvedené údaje mění s gestačním věkem. Hodláme navrhnout predikční modely pro odhad rizika GDM na základě hladin steroidů v krvi u žen v těhotenství i mimo něj a dále i případné modifikace léčby GDM.
Scientific branches
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
The project contributed to the acquisition of basic knowledge about the pathophysiology of gestational diabetes and the role of the steroid metabolome in its development. The robust GC-MS / MS methodology enabling the determination of 100 conjugated and unconjugated metabolites offers the potential for diagnostic and prognostic / predictive use in a wider range of pathologies. A valuable result is modeling the risk of developing intrahepatic cholestasis and recognizing the importance of the relationship between testosterone levels and endocrine disruptors in umbilical cord blood in the development of the male fetus. The results of the project were adequately presented, including 10 original papers in journals with IF. Overall, the project can be assessed as successful. Its contribution to the education of postgraduate students and other experts, especially in the field of analytical chemistry, is not negligible.
Solution timeline
Realization period - beginning
Apr 1, 2017
Realization period - end
Dec 31, 2021
Project status
U - Finished project
Latest support payment
Mar 19, 2020
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP22-MZ0-NV-U
Data delivery date
Jun 30, 2022
Finance
Total approved costs
11,201 thou. CZK
Public financial support
11,154 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
47 thou. CZK
Basic information
Recognised costs
11 201 CZK thou.
Public support
11 154 CZK thou.
99%
Provider
Ministry of Health
CEP
FB - Endocrinology, diabetology, metabolism, nutrition
Solution period
01. 04. 2017 - 31. 12. 2021