Role of Mmi1 in redistribution of Cdc14 phosphataseand stress-induced fragmentation of mitochondria
Public support
Provider
Ministry of Education, Youth and Sports
Programme
—
Call for proposals
FP6-2002-Mobility-10
Main participants
Mikrobiologický ústav AV ČR, v. v. i.
Contest type
RP - Co-financing of EC programme
Contract ID
MSMT-502/2016-1
Alternative language
Project name in Czech
Role of Mmi1 in redistribution of Cdc14 phosphataseand stress-induced fragmentation of mitochondria
Annotation in Czech
There is growing evidence that in times of stress there is an intense cross-talk between mitochondria and the nucleus. This cross-talk leads to a “life or death” decision. Yeast protein Mmi1 is a homolog of the mammalian "translationally controlled tumor protein" (TCTP), which plays a critical role in controlling cell survival. We found that in heat-stress cells Mmi1 relocalizes into the nucleus, but also associates with stress granules and mitochondria. We proved that Mmi1 significantly affects proteasome degradation. Our preliminary data revealed that Mmi1 affects distribution of phosphatase Cdc14 and possibly also the function of Cdc14 in controlling the cell cycle and stress induced fragmentation of mitochondria. In this project we would like to address a function of Mmi1 in controlling Cdc14 fate and functions. We aim to elucidate the following questions: What is a role of Mmi1 in controlling Cdc104 redistribution upon normal growth conditions and heat-stress at 40°C? We assume that results from the study on yeast will contribute to better understanding general aspects of a TCTP function in and stress survival and cell fate controls of any eukaryotic cell.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EB - Genetics and molecular biology
CEP - secondary branch
CE - Biochemistry
CEP - another secondary branch
EA - Morphology and cytology
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10601 - Cell biology<br>10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology<br>10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>30101 - Human genetics
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
This project was being realized in the framework of the MOBILITY Activity that aims primarily on establishing and strenghtening ties with foreign research institutions. The control of particular outputs is not implemented by the evalution committee, but the correctness of allocated finances and the adequacy of their use are checked.
Solution timeline
Realization period - beginning
Jan 1, 2016
Realization period - end
Dec 31, 2017
Project status
U - Finished project
Latest support payment
Feb 22, 2017
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP18-MSM-7A-U/02:1
Data delivery date
Jun 8, 2018
Finance
Total approved costs
152 thou. CZK
Public financial support
152 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK