Mechanical Disruption of Organelles Shapes Liver Cancer Cell Fate at the Subcellular Level
Public support
Provider
Czech Science Foundation
Programme
Standard projects
Call for proposals
SGA0202600001
Main participants
Fyzikální ústav AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
26-21666S
Alternative language
Project name in Czech
Mechanical Disruption of Organelles Shapes Liver Cancer Cell Fate at the Subcellular Level
Annotation in Czech
Mechanical cues from both the extracellular environment and intracellular structures play a crucial role in liver cancer. In conditions like fibrosis and hepatocellular carcinoma (HCC), increased tissue stiffness affects cellular processes such as migration, proliferation, and chemoresistance. Lipid droplets (LDs), key modulators of cellular stress, contribute to nuclear deformation, cytoskeletal reorganization, and metabolic disruption, though the mechanisms remain unclear. This project investigates the impact of extracellular (substrate stiffness) and intracellular (LD-induced stress) forces on liver cancer cell function at the single-cell and sub-cellular levels. We hypothesize that mechanical disruption of organelles, particularly via LDs, alters cellular fate by affecting gene expression, organelle positioning, and biophysical processes. Using advanced 2D and 3D cell cultures, we aim to uncover how LD-driven mechanical stress modulates liver cancer cell functions and reveal potential therapeutic targets for HCC progression.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10610 - Biophysics
OECD FORD - secondary branch
10601 - Cell biology
OECD FORD - another secondary branch
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CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
BO - Biophysics<br>EA - Morphology and cytology
Solution timeline
Realization period - beginning
Jan 1, 2026
Realization period - end
Dec 31, 2028
Project status
Z - Beginning multi-year project
Latest support payment
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Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP26-GA0-GA-R
Data delivery date
Jun 8, 2026
Finance
Total approved costs
10,596 thou. CZK
Public financial support
10,302 thou. CZK
Other public sources
294 thou. CZK
Non public and foreign sources
0 thou. CZK