3D yeast colony genomics: A model for cancer progression and development of drug resistance in biofilms
Public support
Provider
Ministry of Education, Youth and Sports
Programme
EEA/Norwegian Financial Mechanism
Call for proposals
Finanční mechanismy EHP/Norsko 1 (SMSM20147F1)
Main participants
Univerzita Karlova / Přírodovědecká fakulta
Contest type
VS - Public tender
Contract ID
MSMT-28477/2014
Alternative language
Project name in Czech
3D yeast colony genomics: A model for cancer progression and development of drug resistance in biofilms
Annotation in Czech
Studies of cell differentiation, spatial position, cell-cell interaction and signaling within organized yeast communities such as colonies and biofilms have revealed the existence of unique processes associated with the yeast multicellular life-style. These findings showed that specific attributes attributed previously only to metazoans have their counterparts in yeast multicellular structures. Yeast colonies therefore recently became an excellent simple model for investigation of these processes, the molecular principles of which are often conserved among eukaryotes, including humans. Recent findings of both Czech teams behind this proposal showed that colonies behave as simple multicellular organisms in which cells differentiate, acquire specific properties and communicate with each other. The main aim of the project is to identify, using next-generation DNA/RNA sequencing methodology provided by the Norwegian partner, novel molecular mechanisms involved in development of yeast cells within differentiated colony resembling cells of mammalian solid tumors, as well as those involved in development of protective mechanisms in yeast biofilm colonies. The research is based on recent findings of both Czech partners regarding metabolic parallels between differentiated yeast colonies and tumor-affected organism and of those regarding defense strategies in biofilm colonies. YEASTSEQ will also create new long-term cooperation between Czech and Norwegian teams and significantly expand the knowledge and skills of the participating teams, including PhD students and young researchers. Specific aims include: i) Quantitative analysis of transcriptomes of different types of differentiated cells of yeast colonies by RNA Seq; ii) identification of the mutation rate in the cell subpopulations by whole genome re-sequencing and iii) determining of epigenetic regulations involved in the reprogramming of structured biofilm colonies by FAIRE and Chromatin Immunoprecipitation (ChIP).
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EB - Genetics and molecular biology
CEP - secondary branch
EE - Microbiology, virology
CEP - another secondary branch
FD - Oncology and haematology
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10603 - Genetics and heredity (medical genetics to be 3)<br>10604 - Reproductive biology (medical aspects to be 3)<br>10605 - Developmental biology<br>10606 - Microbiology<br>10607 - Virology<br>10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>30101 - Human genetics<br>30204 - Oncology<br>30205 - Hematology
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
The YEASTSEQ project may be deemed executed but it did not publish any outcomes during its eligible time. Nevertheless, the results reveal basic traits of tumor-like yeast cells which could enable further studies of medically important pathogenic yeast films. The overall project rating is good.
Solution timeline
Realization period - beginning
Oct 15, 2014
Realization period - end
Apr 30, 2017
Project status
U - Finished project
Latest support payment
Mar 15, 2017
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP18-MSM-7F-U/05:1
Data delivery date
Nov 21, 2018
Finance
Total approved costs
25,076 thou. CZK
Public financial support
25,076 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK