The role of pH in the development and differentiation of multicellular yeast populations
Public support
Provider
Ministry of Education, Youth and Sports
Programme
INTER-EXCELLENCE
Call for proposals
SMSM2020LTC01
Main participants
Univerzita Karlova / Přírodovědecká fakulta
Contest type
VS - Public tender
Contract ID
MSMT-21080/2020-19
Alternative language
Project name in Czech
The role of pH in the development and differentiation of multicellular yeast populations
Annotation in Czech
Project of international cooperation in the frame of COST Action EuroMicropH “Understanding and exploiting the impact of low pH on microorganisms” aims to develop new approaches for in situ, intracellular pH measurement in multicellular yeast populations - colonies and biofilms, and to uncover the effect of intracellular pH in regulating processes involved in colony development and yeast cell differentiation. The project also aims to acquire new knowledge regarding the effect of intracellular pH fluctuations on yeast cell adhesion to plastic surfaces and formation of structured biofilms. The project will utilize yeast populations (colonies and solid-liquid-interface biofilms) cultivated under defined conditions that facilitate investigation of processes related to metabolic reprogramming and cell differentiation with regard to changes in intracellular H+ (pH) therein. The project builds on previous, ground-breaking findings of both Czech teams, who i) identified specific processes involved in the development and differentiation of yeast colonies (including associated regulations), ii) identified novel mechanisms involved in adhesion of yeast cells to plastic, and iii) implemented a new method of pH measurement, based on wavelength shifts of pH sensitive fluorophores. The complementary know-how of participating teams and interaction with COST Action research groups with diverse expertise (including medical applications) will have a synergistic effect on research speed and effectiveness and will create a knowledge base, leading to further extension of the research and potential future follow-up projects, including application projects. The project will yield publications in prestigious foreign scientific journals.
Scientific branches
R&D category
ZV - Basic research
OECD FORD - main branch
10606 - Microbiology
OECD FORD - secondary branch
10607 - Virology
OECD FORD - another secondary branch
10605 - Developmental biology
CEP - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
EB - Genetics and molecular biology<br>EE - Microbiology, virology
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
Research on pH role in development and differentiation of yeast populations continued as planned. We developed new methods to detect changes in intracellular pH within colonies and identified changes mainly in the cytosol and vacuole during their development. Prepared publications present relationships between pH, metabolic changes, and the function of selected regulators during colony development.
Solution timeline
Realization period - beginning
May 15, 2020
Realization period - end
May 16, 2023
Project status
U - Finished project
Latest support payment
Feb 17, 2023
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP24-MSM-LT-U
Data delivery date
Jul 1, 2024
Finance
Total approved costs
7,285 thou. CZK
Public financial support
7,285 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK