The role of mitochondrial energy metabolism and redox regulations in pulmonary hypertension
Public support
Provider
Ministry of Education, Youth and Sports
Programme
KONTAKT II
Call for proposals
KONTAKT II 5 (SMSM2015LH5)
Main participants
Fyziologický ústav AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
MSMT-32267/2015-1
Alternative language
Project name in Czech
Role mitochondriálního energetického a redoxního metabolismu v rozvoji plicní hypertenze
Annotation in Czech
The proposed project will study the detailed role of mitochondrial bioenergetics and redox regulations in initiation and development of pulmonary hypertension induced by chronic hypoxia. We will use the unique biological model, i.e. calf and human fibroblasts derived from pulmonary arteries. The study deals with nowadays widely respected metabolic theory of pulmonary hypertension. We will focus on mitochondria as the principal powerhouse and redox regulator of cellular metabolism. We will uncover changes in substrate distribution for mitochondrial bioenergetics; the role and regulation of suppressed respiratory Complex I activity; the role of reactive oxygen species originated in mitochondria vs. cytosolic compartment and the role of mitochondrial morphology changes in calf and human fibroblasts derived from animals/humans with developed pulmonary hypertension. The uncovered knowledge will be valuable in future for designing of proper therapy for people with pulmonary hypertension.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
ED - Physiology
CEP - secondary branch
FC - Pneumology
CEP - another secondary branch
EA - Morphology and cytology
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10601 - Cell biology<br>10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology<br>30105 - Physiology (including cytology)<br>30203 - Respiratory systems
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
We uncovered regulatory pathways of energy metabolism and redox state ofpulmonary arterial fibroblasts. Their changes lead to development of pulmonary hypertension. Affected fibroblasts increase their glycolysis vs. mitochondrial oxidation , show prooxidative metabolism, all causing high proliferation and proinflammatory status leading to remodelation of cell wall.
Solution timeline
Realization period - beginning
Jan 1, 2016
Realization period - end
Dec 31, 2017
Project status
U - Finished project
Latest support payment
Mar 2, 2017
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP18-MSM-LH-U/01:1
Data delivery date
Jun 7, 2018
Finance
Total approved costs
1,726 thou. CZK
Public financial support
1,726 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK