Contribution of superresolution microscopy and image analysis to the study of plant in vitro cultures with the emphasis on somatic embryogenesis of conifers
Public support
Provider
Ministry of Education, Youth and Sports
Programme
INTER-EXCELLENCE
Call for proposals
INTER-EXCELLENCE 2 (SMSM2016LTC01)
Main participants
Ústav experimentální botaniky AV ČR, v. v. i.
Contest type
VS - Public tender
Contract ID
MSMT-15304/2017-1
Alternative language
Project name in Czech
Přínos mikroskopie s vysokým rozlišením a analýzy obrazu ke studiu rostlinných in vitro kultur, zejména embryogenní kultury jehličnanů
Annotation in Czech
The aim of the COST Action CA15124 - A new Network of European BioImage Analysts to advance life science imaging (NEUBIAS) is to maximise the outputs of image-based research in life sciences by empowering the international network of bioimage analysis (IA) experts - BIAlysts. The Action will organize meetings to foster interactions of all stakeholders including life scientists, bialysts, microscopists, software developers and private sector. The aim of our project is to create IA methods of microscopic/IA tasks which we have met during the study of our main research topic – somatic embryogenesis of conifers. We will estimate the volume of non-embryogenic mass in somatic embryogenic culture of Douglas fir by the combination of Cavalieri´s principle and the point-counting method. We will use laser scanning confocal microscope to quantify viability of the cells of Norway spruce early somatic embryos. Using measurements of optical parameters and colour characteristics, we will quantify histochemical proof of phenolic compounds on the Norway spruce somatic embryo sections. Together with bialysts we will try to quantify immunohistochemical reaction on the Norway spruce somatic embryo sections and the protein turnover during starvation-dependent autophagy process using photoconvertible proteins in tobacco BY-2 cells. We provide our datasets to the interactive database for bioimage analysis tools and workflows that will be created by the Action.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
EF - Botany
CEP - secondary branch
ED - Physiology
CEP - another secondary branch
EA - Morphology and cytology
OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
10601 - Cell biology<br>10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology<br>10611 - Plant sciences, botany<br>10612 - Mycology<br>30105 - Physiology (including cytology)
Completed project evaluation
Provider evaluation
V - Vynikající výsledky projektu (s mezinárodním významem atd.)
Project results evaluation
We prepared workflows for evaluation of microscopic images of the histological plant tissue sections, plant cells observed under DIC and fluorescent images of whole plant cells labelled with antibodies. We evaluated images of three plant models – embryogenic cultures of Douglas-fir and Norway spruce and tobacco cell culture BY-2. For evaluation of images we used methods of digital image analysis and tools of programs Ellipse, NIS-Elements and ImageJ.
Solution timeline
Realization period - beginning
May 17, 2017
Realization period - end
May 2, 2020
Project status
U - Finished project
Latest support payment
Feb 24, 2020
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP21-MSM-LT-U/01:1
Data delivery date
Jun 15, 2021
Finance
Total approved costs
2,545 thou. CZK
Public financial support
2,545 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK