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The histological microstructure and in vitro mechanical properties of pregnant and postmenopausal ewe perineal body

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023698%3A_____%2F19%3AN0000019" target="_blank" >RIV/00023698:_____/19:N0000019 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11120/19:43918740 RIV/00216208:11140/19:10402400 RIV/49777513:23520/19:43957151 RIV/00669806:_____/19:10402400

  • Result on the web

    <a href="https://doi.org/10.1097/GME.0000000000001395" target="_blank" >https://doi.org/10.1097/GME.0000000000001395</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1097/GME.0000000000001395" target="_blank" >10.1097/GME.0000000000001395</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The histological microstructure and in vitro mechanical properties of pregnant and postmenopausal ewe perineal body

  • Original language description

    The mechanical properties and microstructure of the perineal body are important for the improvement of numerical models of pelvic organs. We determined the mechanical parameters and volume fractions of the ewe perineal body as an animal model. METHODS: The 39 specimens of 13 pregnant swifter ewes delivering by cesarean section (aged 2 years, weight 61.2 +- 6.2 kg (mean +- standard deviation) and 24 specimens of 8 postmenopausal swifter ewes 150 days after surgical ovariectomy (aged 7 years, 58.6 +- 4.6 kg)) were loaded uniaxially to determine Young's moduli of elasticity in the small (E0) and large (E1) deformation regions, and ultimate stresses and strains. The 63 adjacent tissue samples were processed histologically to assess volume fractions of smooth and skeletal muscle, adipose cells, elastin, and type I collagen using a stereological point testing grid. We compared the structural and mechanical differences along the ewe perineal body, and between pregnant and postmenopausal groups. RESULTS: The pregnant/postmenopausal perineal body was composed of smooth muscle (12/14%; median), skeletal muscle (12/16%), collagen (10/23%), elastin (8/7%), and adipose cells (6/6%). The E0 was 37/11 kPa (median), E1 was 0.97/1.04 MPa, ultimate stress was 0.55/0.59 MPa, and ultimate strain was 0.90/0.87 for pregnant/postmenopausal perineal body. The perineal body showed a structural and mechanical stability across the sites. The pregnant ewes had a higher amount of skeletal muscle, higher E0, and a less amount of collagen when compared with postmenopausal ewes. CONCLUSIONS: The data can be used as input for models simulating vaginal delivery, pelvic floor prolapsed, or dysfunction.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30214 - Obstetrics and gynaecology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Menopause

  • ISSN

    1072-3714

  • e-ISSN

    1530-0374

  • Volume of the periodical

    26

  • Issue of the periodical within the volume

    11

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    13

  • Pages from-to

    1289-1301

  • UT code for WoS article

    000509483500011

  • EID of the result in the Scopus database

    2-s2.0-85072100817