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Actinomycin D upregulates proapoptotic protein Puma and downregulates Bcl-2 mRNA in normal peripheral blood lymphocytes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F07%3A00007107" target="_blank" >RIV/00023736:_____/07:00007107 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Actinomycin D upregulates proapoptotic protein Puma and downregulates Bcl-2 mRNA in normal peripheral blood lymphocytes

  • Original language description

    In PBL cultured in vitro the apoptosis induced by concentration of ActD causing almost exclusively damage of rDNA and subsequent ribosomal stress is dependent of p53 and Puma activation. The Bcl-2 mRNA downregulation is dependent of nucleolin cleavage byexecutive caspases. This apoptotic way, demonstrated in peripheral blood lymphocytes for the first time, is not the only way inducible in PBL by this cytostatic agent and by other genotoxic drugs

  • Czech name

    Aktinomycin D indukuje proapoptotický protein Puma a reprimuje Bcl-2 mRNA v normálních periferních krevních lymfocytech

  • Czech description

    V periferních krevních lymfocytech apoptoza indukovaná aktinomycinem D a následným ribozomálním stresem závisí na aktivaci p53 a Puma proteinů. Bcl-2 mRNA je redukována díky štěpení nukleolinu exekutivními kaspázami. Tato apoptotická cesta není jediná indukovatelná v lymfocytech aktinomycinem D či jinými genotoxickými látkami

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FD - Oncology and haematology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/NR7998" target="_blank" >NR7998: Induction of processes blocking the proliferation of leukemic cells by inhibition of transcription of ribosomal genes.</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2007

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Anti-Cancer Drugs

  • ISSN

    0959-4973

  • e-ISSN

  • Volume of the periodical

    18

  • Issue of the periodical within the volume

    7

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    10

  • Pages from-to

    763-772

  • UT code for WoS article

  • EID of the result in the Scopus database