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Partial molecular characterization of a Czech isolate of Grapevine leafroll-associated virus 3

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A1248" target="_blank" >RIV/00027006:_____/04:1248 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Partial molecular characterization of a Czech isolate of Grapevine leafroll-associated virus 3

  • Original language description

    Grapevine leafroll-associated virus 3 (GLRaV-3) is an important pathogen of grapevine in the Czech Republic. A Czech isolate was studied at the molecular level in order to extend data on molecular variability of the virus from the central European region. Three conserved regions (genes encoding the RNA-dependent RNA polymerase, the heat shock protein 70 and the 55 kDa protein) were amplified using reverse transcriptase - polymerase chain reaction, sequenced and compared with related available sequences.On the sequenced regions, the Czech isolate revealed more than 99% nucleotide and amino acid identity with the published full-length sequence of a North American GLRaV-3 isolate and a similarly high identity with available partial GLRaV-3 sequences of isolates from different parts of the world.

  • Czech name

    Částečná charakterizace českého izolátu viru svinutky révy 3 na molekulární úrovni

  • Czech description

    Virus spojený se svinutkou révy vinné 3 (Grapevine leafroll-associated virus 3 - GLRaV-3) je v České republice závažným patogenem révy vinné. Český izolát tohoto viru byl studován na molekulární úrovni, abychom získali poznatky o molekulární variabilitětohoto viru v oblasti střední Evropy. Byly vybrány tři konzervativní oblasti - gen pro RNA polymerázu, gen pro heat-shock protein 70 a gen pro 55 kDa protein. Tyto tři oblasti byly amplifikovány pomocí reverzní transkripce a polymerázové řetězové reakce,osekvenovány a porovnány s dostupnými sekvencemi. Ve zkoumaných oblastech se český izolát shodoval více než z 99% s publikovanou celou sekvencí severoamerického izolátu tohoto viru. Podobně vysokou identitu měl český izolát s dostupnými částečnými sekvencemi GLRaV-3 z různých částí světa.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GF - Diseases, pests, weeds and plant protection

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GP522%2F01%2FD131" target="_blank" >GP522/01/D131: Characterization of grapevine leafroll - associated closteroviruses in the territory of the Czech republic.</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Phytopathology

  • ISSN

    0931-1785

  • e-ISSN

  • Volume of the periodical

    152

  • Issue of the periodical within the volume

    7

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    5

  • Pages from-to

    427-431

  • UT code for WoS article

  • EID of the result in the Scopus database