Comparison of four techniques for plum pox virus detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003747" target="_blank" >RIV/00027006:_____/16:00003747 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43510/16:43909728
Result on the web
<a href="http://dx.doi.org/10.1007/s41348-016-0043-y" target="_blank" >http://dx.doi.org/10.1007/s41348-016-0043-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s41348-016-0043-y" target="_blank" >10.1007/s41348-016-0043-y</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of four techniques for plum pox virus detection
Original language description
Twenty-four stone fruit trees showing typical symptoms of plum pox virus (PPV) were tested for PPV using ELISA, RT-PCR, real-time RT-PCR and RT-LAMP. The isolates were obtained from various regions in the Czech Republic. PPV was detected in all tested plants. Part of the coding region of the PPV coat protein gene and adjacent non-coding region were sequenced. PPV strains of individual isolates were classified according to the obtained sequences. This study provides comprehensive evaluation of the sensitivity of four techniques used for PPV detection. Five PPV strains (D, M, W, Rec and An) were observed according to analyses of obtained sequences. The results showed that the degree of sensitivity for each detection technique varied across the four chosen methods, with the RT-LAMP technique providing very sensitive results.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Plant Diseases and Protection
ISSN
1861-3829
e-ISSN
—
Volume of the periodical
123
Issue of the periodical within the volume
6
Country of publishing house
DE - GERMANY
Number of pages
5
Pages from-to
311-315
UT code for WoS article
000392201900008
EID of the result in the Scopus database
—