Grey poplar explant acclimation to improve the dehydration tolerance and cryopreservation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10149485" target="_blank" >RIV/00027006:_____/20:10149485 - isvavai.cz</a>
Alternative codes found
RIV/00020702:_____/20:N0000062
Result on the web
<a href="https://bp.ueb.cas.cz/pdfs/bpl/2020/01/28.pdf" target="_blank" >https://bp.ueb.cas.cz/pdfs/bpl/2020/01/28.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.32615/bp.2019.148" target="_blank" >10.32615/bp.2019.148</a>

Result language
angličtina
Original language name
Grey poplar explant acclimation to improve the dehydration tolerance and cryopreservation
Original language description
Cryostorage techniques have been developed to preserve the most valuable genotype from an endangered native population of Populus x canescens Aiton Sm. (grey poplar), which is located in the floodplain forest in the South Moravia region of the Czech Republic and which is difficult to propagate using cuttings. The prevailing genotype with valuable traits was selected by the simple sequence repeats method. This genotype was used to determine the most effective pre-cultivation conditions (cold hardening, and cold hardening combine with osmotic treatment) on dehydration tolerance and post-thaw recovery of the grey poplar shoot tips. The pre-cultivation and application of a modified plant vitrification solution 3 (PVS3) considerable reduced the freezable water content in shoot tips. Evaluation after eight weeks of regrowth revealed that simultaneous effect of cold and osmotic pre-treatments and application of PVS3 for 120 min enabled 93.3 +/- 5.8 % recovery. The results emphasize the importance of the pre-cultivation conditions, which significantly improved the post-thaw recovery of the grey poplar explants.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40102 - Forestry

Project
<a href="/en/project/QK1910277" target="_blank" >QK1910277: The use of cryopreservation method to streamline breeding process of economically important agricultural crops and preservation of forest tree</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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