Investigation of the Effect of the Auxin Antagonist PEO-IAA on Cannabinoid Gene Expression and Content in Cannabis sativa L. Plants under In Vitro Conditions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176159" target="_blank" >RIV/00027006:_____/23:10176159 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/23:73620328 RIV/61989592:15640/23:73620328

Result on the web

<a href="https://www.mdpi.com/2223-7747/12/8/1664/pdf?version=1681550868" target="_blank" >https://www.mdpi.com/2223-7747/12/8/1664/pdf?version=1681550868</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/plants12081664" target="_blank" >10.3390/plants12081664</a>

Result language

angličtina

Original language name

Investigation of the Effect of the Auxin Antagonist PEO-IAA on Cannabinoid Gene Expression and Content in Cannabis sativa L. Plants under In Vitro Conditions

Original language description

The in vitro shoot propagation of Cannabis sativa L. is an emerging research area for large-scale plant material production. However, how in vitro conditions influence the genetic stability of maintained material, as well as whether changes in the concentration and composition of secondary metabolites can be expected are aspects that need to be better understood. These features are essential for the standardised production of medicinal cannabis. This work aimed to find out whether the presence of the auxin antagonist alpha-(2-oxo-2-phenylethyl)-1H-indole-3-acetic acid (PEO-IAA) in the culture media influenced the relative gene expression (RGE) of the genes of interest (OAC, CBCA, CBDA, THCA) and the concentrations of studied cannabinoids (CBCA, CBDA, CBC, increment (9)-THCA, and increment (9)-THC). Two C. sativa cultivars, &apos;USO-31&apos; and &apos;Tatanka Pure CBD&apos;, were cultivated by in vitro conditions with PEO-IAA presence and then analysed. The RT-qPCR results indicated that even though some changes in the RGE profiles could be observed, no differences were statistically significant compared with the control variant. The results of the phytochemical analyses demonstrate that although there were some differences from the control variant, only the cultivar &apos;Tatanka Pure CBD&apos; showed a statistically significant increase (at a statistical significance level alpha = 0.05) in the concentration of the cannabinoid CBDA. In conclusion, it would appear that using PEO-IAA in the culture medium is a suitable approach to improve in vitro cannabis multiplication.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/EF19_073%2F0016713" target="_blank" >EF19_073/0016713: Improving schematics of Doctoral student grant competition and their pilot implementation</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plants-Basel

ISSN

2223-7747

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

8

Country of publishing house

CH - SWITZERLAND

Number of pages

17

Pages from-to

1664

UT code for WoS article

000979043300001

EID of the result in the Scopus database

2-s2.0-85156264669