Gene expression in the chicken caecum is dependent on microbiota composition

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000098" target="_blank" >RIV/00027162:_____/17:N0000098 - isvavai.cz</a>

Result on the web

<a href="https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-017-0493-7" target="_blank" >https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-017-0493-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13567-017-0493-7" target="_blank" >10.1186/s13567-017-0493-7</a>

Result language

angličtina

Original language name

Gene expression in the chicken caecum is dependent on microbiota composition

Original language description

Gut microbiota is of considerable importance for each host. Despite this, germ-free animals can be obtained and raised to sexual maturity and consequences of the presence or absence of gut microbiota on gene expression of the host remain uncharacterised. In this study, we performed an unbiased study of protein expression in the caecum of germ-free and colonised chickens. The major difference between these two groups was in the expression of immunoglobulins which were essentially absent in the germ-free chickens. Microbiota also caused a minor decrease in the expression of focal adhesion and extracellular matrix proteins and an increase in the expression of argininosuccinate synthase ASS1, redox potential sensing, fermentative metabolic processes and detoxification systems represented by sulfotransferases SULT1C3 or SULT1E1. Since we also analysed expression in the caecum of E. coli Nissle and E. faecium DSM7134 mono-associated chickens, we concluded that at least immunoglobulin expression and expression of cystathionine synthase (CBS) was dependent on microbiota composition with E. coli Nissle stimulating more immunoglobulin and PIGR expression and E. faecium DSM7134 stimulating more CBS expression. Gut microbiota and its composition therefore affected protein expression in the chicken caecum though except for immunoglobulin production, the remaining differences were unexpectedly low.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Research

ISSN

0928-4249

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

48

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

85

UT code for WoS article

000417072700001

EID of the result in the Scopus database

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