Dynamics of mono- and dual-species biofilm formation and interactions between Staphylococcus aureus and Gram-negative bacteria

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000112" target="_blank" >RIV/00027162:_____/17:N0000112 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1111/1751-7915.12705/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/1751-7915.12705/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1751-7915.12705" target="_blank" >10.1111/1751-7915.12705</a>

Result language

angličtina

Original language name

Dynamics of mono- and dual-species biofilm formation and interactions between Staphylococcus aureus and Gram-negative bacteria

Original language description

Microorganisms are not commonly found in the planktonic state but predominantly form dual- and multispecies biofilms in almost all natural environments. Bacteria in multispecies biofilms cooperate, compete or have neutral interactions according to the involved species. Here, the development of mono- and dual-species biofilms formed by Staphylococcus aureus and other foodborne pathogens such as Salmonella enterica subsp. enterica serovar Enteritidis, potentially pathogenic Raoultella planticola and non-pathogenic Escherichia coli over the course of 24, 48 and 72h was studied. Biofilm formation was evaluated by the crystal violet assay (CV), enumeration of colony-forming units (CFUcm(-2)) and visualization using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). In general, Gram-negative bacterial species and S.aureus interacted in a competitive manner. The tested Gram-negative bacteria grew better in mixed dual-species biofilms than in their mono-species biofilms as determined using the CV assay, CFUml(-2) enumeration, and CLSM and SEM visualization. In contrast, the growth of S.aureus biofilms was reduced when cultured in dual-species biofilms. CLSM images revealed grape-like clusters of S.aureus and monolayers of Gram-negative bacteria in both mono- and dual-species biofilms. S.aureus clusters in dual-species biofilms were significantly smaller than clusters in S.aureus mono-species biofilms.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microbial Biotechnology

ISSN

1751-7915

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

819-832

UT code for WoS article

000404123100016

EID of the result in the Scopus database

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