The Use of Confocal and Two Photon Excitation Microscopy to Study Testate Amoebae

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F08%3A12148202" target="_blank" >RIV/68407700:21460/08:12148202 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The Use of Confocal and Two Photon Excitation Microscopy to Study Testate Amoebae

Original language description

Testate amoebae (Protozoa:Rhizopoda) are a group of unicellular animals (20 - 400 ?m), protected with the shell (SiO2, CaCO3, proteins), living in fresh water habitats. They are used as model organisms in population ecology, ecotoxicology, paleoecology -thanks to their cosmopolitan disperse and species-specific ecological preferences together with low ecological valence to the changes of the environmental surroundings. Testate amoebae are usually examined using scanning electron microscopy (SEM) and environmental SEM (E-SEM). Scanning electron microscope allows for much better resolution than confocal laser scanning microscopy (CLSM) far sufficient for recognizing determinant features), but is limited to viewing surface of the specimen. For this reason, we use CLSM and two-photon excitation (TPE) fluorescence microscopy to visualize the shell and inner structures of testate amoebae. We tried as many as 15 fluorescent dyes.

Czech name

Použití konfokální a dvoufotonové excitační mikroskopie pro studium Testate Amoebae

Czech description

Použili jsme laserový konfokální rastrovací mikroskop a dvoufotonovou excitační fluorescenční mikroskopii pro studium schránky a vnitřních struktur Testate Amoebae (krytenek). Použili jsme 15 fluorescenčních barviček.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EH - Ecology - communities

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological Research

ISSN

0862-8408

e-ISSN

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Volume of the periodical

53

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

2

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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